基于网络药理学和蛋白模块探讨培土清心颗粒治疗特应性皮炎的作用机制

目的运用网络药理学和蛋白模块方法探讨培土清心颗粒治疗特应性皮炎(atopic dermatitis,AD)的作用机制。方法基于中药系统药理学数据库及分析平台(TCMSP)和BATMAN-TCM数据库获取培土清心颗粒化学成分及其对应靶点,通过GeneCards、OMIM、DisGeNET数据库筛选特应性皮炎疾病靶点。使用String数据库构建蛋白相互作用(PPI)网络;使用Cytoscape软件绘制培土清心颗粒潜在活性成分-靶点-疾病网络图,使用Network Analyzer工具对网络进行拓扑属性分析;以插件ClusterMaker对靶点进行模块化分解,并使用DAVID数据库进行基因百科全书(KEGG)通路富集分析。结果得到培土清心颗粒治疗特应性皮炎活性成分185个,靶点125个,重要活性成分包括檞皮素、木犀草素、山奈酚、汉黄芩素等;重要靶点包括PTGS2、CXCL8、IL-10、CCL2等;PPI网络模块化分析结果显示,125个靶点主要分为3个模块,KEGG通路富集方面,模块一内靶点KEGG通路主要与特应性皮炎免疫系统、炎症反应相关;模块二内靶点KEGG通路主要与细胞增殖、分化、凋亡相关;模块三内靶点KEGG通路与代谢密切相关。结论培土清心颗粒可能通过抗炎、调节免疫、调控细胞凋亡以及调节氨基酸代谢以达到治疗特应性皮炎的目的。

Study on the Mechanism of Peitu Qingxin Granules in the Treatment of Atopic Dermatitis Based on Network Pharmacology and Protein Module

Objective To explore the mechanism of Peitu Qingxin granules in the treatment of atopic dermatitis(atopic dermatitis,AD)by means of network pharmacology and protein module.Methods The chemical constituents and corresponding targets of Peitu Qingxin granules were obtained based on the traditional Chinese medicine system pharmacology database and analysis platform(TCMSP)and BATMAN-TCM database,and the disease targets of AD were screened by Gene Cards,OMIM and DisGeNET databases.String database was used to construct protein interaction(PPI)network.Cytoscape software was used to draw the potential active component-target-disease network map of Peitu Qingxin granules.We analyzed the topological properties of the network by Network Analyzer tool.Then,plug-in ClusterMaker was used to modularize the targets,and DAVID database was used for Kyoto Encyclopedia of Gene and Genomes(KEGG)pathway enrichment analysis.Results 185 active components and 125 targets of Peitu Qingxin granules were obtained.Quercetin,luteolin,kaempferol and wogonin were the main ingredients in Peitu Qingxin granules.And the key targets were PTGS2,CXCL8,IL-10 and CCL2.The results of PPI network modularization analysis showed that the 125 targets were mainly divided into three modules.In the aspect of KEGG pathway enrichment,targets are mainly related to AD immune system and inflammation in module one,the pathways of module two targets are mainly related to cell proliferation,differentiation and apoptosis,and targets are closely related to metabolism in module three.Conclusion Peitu Qingxin granules may treat AD by antiinflammation,regulating immunity,regulating apoptosis and regulating amino acid metabolism.