基于高通量测序技术的黄芩提取物对热证模型大鼠肠道菌群多样性的影响

目的 探究黄芩Scutellaria baicalensis提取物对热证模型大鼠肠道菌群多样性的影响,并探讨中医"热者寒之"思想的科学内涵.方法 雄性SD大鼠随机分为对照组、模型组、黄芩提取物(3 g/kg)组.除对照组外,其余各组大鼠于每日上午ig左甲状腺素钠混悬液(0.12 g/kg),造模lh后,黄芩提取物组ig...

Effect of Scutellaria baicalensis extract on intestinal microflora diversity in rats with heat syndrome based on high-throughput sequencing

Objective To explore the effect of Scutellaria baicalensis extract on the diversity of intestinal flora in heat syndrome model rats,and explore the scientific connotation of the thought of“treating heat syndrome with cold natured drugs”in traditional Chinese medicine.Methods Male SD rats were randomly divided into control group,model group and S.Baicalensis extract(3 g/kg)group.Except for the control group,rats in the other group were ig levothyroxine sodium suspension(0.12 g/kg)every morning,one hour after modeling,rats in S.baicalensis extract group were ig S.baicalensis extract(10 mL/kg),rats in control group and model group were ig same volume of drinking water for 15 d.16 S rRNA technique was used to sequence the intestinal microflora in the V4 area of the cecum,and the rat serum was taken to determine 12 key indexes in the neuro-endocrine-immune system.The interaction system between differential bacteria and neuro-endocrine-immune system was constructed.Results S.baicalensis extract significantly adjusted 12 key indexes in the neuro-endocrine-immune system of heat syndrome model rats,significantly improved the number of operational taxonomic units(OTUs)and the imbalance of diversity betweenα,βin the intestinal tract,reduced the biological richness and diversity index of flora,and regulated the relative abundance of six different bacteria and 25 different bacteria in heat model rats.Collinsella,Ochrobactrum and Prevotella had strong correlation with neuro-endocrine-immune system.Conclusion S.baicalensis extract could improve the abnormality of neuro-endocrine-immune system and intestinal flora in heat syndrome model rats.Collinsella,Ochrobactrum and Prevotella may be potential markers of heat syndrome model.