2型囊泡单胺转运体分子探针18F-FP-(+)-DTBZ纵向定量评价2型糖尿病大鼠脑多巴胺能神经元

目的 观察2型囊泡单胺转运体（VMAT2）靶点分子探针¹⁸F-FP-（+）-DTBZ评价2型糖尿病（T2DM）大鼠脑多巴胺能神经元的效果。方法 将12只ZDF大鼠均分为T2DM组（fa/fa基因型）和对照组（fa/+基因型），每组6只。于大鼠3、6和12月龄时行¹⁸F-FP-（+）-DTBZ Micro-PET/CT显像，观察2组大鼠体质量、血糖及脑纹状体标准摄取比值（SUVR）变化。结果 T2DM组大鼠6月龄时体质量明显高于对照组（P<0.05），而3月和12月龄时2组体质量差异均无统计学意义（P均>0.05）。T2DM组大鼠3月龄时空腹血糖与对照组差异无统计学意义（P>0.05），而6月龄、12月龄时空腹血糖均明显高于对照组（P均<0.05）。T2DM组大鼠3月龄、6月龄时脑纹状体SUVR与对照组差异均无统计学意义（P均>0.05），12月龄时明显低于对照组（P<0.05）。结论 2型糖尿病可致大鼠脑纹状体VMAT2表达降低，进而引起脑多巴胺能神经元功能异常。

Longitudinal quantification evaluation on dopaminergic neurons in brain of type 2 diabetes mellitus rats with vesicular monoamine transporter type 2 molecular probe 18F-FP-(+)-DTBZ

Objective To observe the effect of ¹⁸F-FP-(+)-DTBZ, vesicular monoamine transporter 2 (VMAT2) molecular probe for evaluating dopaminergic neurons in type 2 diabetes mellitus (T2DM) rats. Methods Twelve ZDF rats were equally divided into T2DM group (fa/fa) and the control group (fa/+)(each n=6). ¹⁸F-FP-(+)-DTBZ Micro-PET/CT imaging was performed when the rats being 3, 6 and 12-month old in both groups to observe the changes of rats'' mass, glucose and the standardized uptake value ratio (SUVR) in the striatum of brains. Results The rats'' mass of those being 6-month old in T2DM group was significantly higher than that in control group (P<0.05), while there was no significant difference between groups for rats being 3 and 12-month old (both P>0.05). The fasting blood glucose of rats being 3-month old was not significant different between groups (P>0.05), while of rats being 6 and 12-month old were significantly higher in T2DM group than in control group (both P<0.05). The striatal SUVR in T2DM group was significantly lower than that of control group for rats being 12-month old (P<0.05), but there was no significant difference between groups for rats being 3 and 6-month old (both P>0.05). Conclusion T2DM could result decrease of VMAT2 expression in the striatum of rat''s brain, which might lead to abnormal function of brain dopaminergic neurons.