基于主成分分析和判别分析对花鹿茸和马鹿茸9类化学成分对比研究

目的 为研究花鹿茸和马鹿茸化学成分的差异,以多种化学成分为指标综合评价花鹿茸和马鹿茸的品质,并通过判别分析试图寻找以化学成分为指标区分花鹿茸和马鹿茸的简单有效的方法.方法 采用多种分析技术方法,测定并比较了花鹿茸、马鹿茸中多糖、粗蛋白、胶原蛋白、硫酸软骨素、氨基酸、脂肪酸、矿质元素、生物胺、核苷9类化学成分的含量差异,...

Comparative study on chemical components of Cervus nippon and Cervus elaphus based on principal component analysis and discriminant analysis

Objective In order to study the differences in chemical constituents, evaluate the quality with multiple chemical components as indicators, and find a simple and effective method to distinguish the antlers from sika deer (Cervus nippon) and red deer (Cervus elaphus). Methods A variety of analytical techniques were used to determine and compare the polysaccharides, crude protein, collagen, chondroitin sulfate, amino acids, fatty acids, mineral elements, biogenic amines, nucleosides of C. nippon and C. elaphus. And SPSS 24.0 was used for principal component analysis (PCA) and discriminant analysis. Results The results showed that the content of polysaccharide, crude protein, amino acid, collagen, fatty acid, mineral element, nucleoside, chondroitin sulfate, and biogenic amine in C. nippon was 9.51, 518.58, 527.74, 223.12, 13.28, 138.36, 2.51, 2.12 mg/g and 70.75 mg/kg; The content in C. elaphus was 8.61, 669.39, 594.84, 258.91, 9.20, 82.62, 1.22, 1.96 mg/g and 136.02 mg/kg. The results of PCA showed that the score of C. nippon was higher than that of C. elaphus. Conclusion In this experiment, the discriminant functions established by 9 chemical components, 17 amino acids, 37 fatty acids, 10 biogenic amines, and 13 nucleosides can classify C. nippon and C. elaphus, indicating that chemical components can be used to distinguish the C. nippon and C. elaphus by discriminant analysis. This study provides a reference for the establishment of quality standards system of C. nippon and C. elaphus in the future.