七叶皂苷钠抗大鼠脑出血后脑水肿作用及其机制

目的探讨七叶皂苷钠(10mg.kg-1.d-1)抗大鼠脑出血后脑水肿的作用及其机制。方法采用♂Wistar大鼠右侧基底核注入50μl自体全血的方法建立模型,并在模型建立后6,24,48或72h处死大鼠。应用干湿重法、酶学方法、免疫组化法和Western blot法,测定各组大鼠脑组织含水量,Na+,K+含量,神经胶质原纤维蛋白(GFAP)阳性细胞和紧密连接相关蛋白occludin表达的变化。结果与假手术组比,模型组出血同侧脑组织含水量,Na+含量和GFAP阳性细胞均明显增加(P<0.05),K+含量和occludin的表达明显减少(P<0.05);与模型组比,治疗组出血同侧脑组织含水量,Na+含量和GFAP阳性细胞明显减少(P<0.05),K+含量和occludin的表达则明显增加(P<0.05)。结论七叶皂苷钠可减轻和(或)延缓脑出血后脑水肿的发生,进而改善脑出血的预后。

Effect and mechanism of sodium aescinate on the brain edema induced by intracerebral hemorrhage in rats

Aim To study the effect of sodium aescinate (10 mg·kg-1·d-1) on brain edema induced by intracerebral hemorrhage and the mechanism. Methods Male Wistar rats received an injection of 50 μlautologous whole blood into the right basal ganglia and were killed 6, 24, 48 or 72 h later. Dry-wet-weighting technique, zymologic, immunohistochemical and Western blot methods were used to examine changes of water content, Na+ and K+ contents, glial fibrillary acidic protein(GFAP)-positive cells and expression of occludin, which is a tight junction(TJ)-associated protein. Results Compared with sham operation group, contents of water and Na+ were significantly higher(P<0.05), the GFAP-positive cells were significantly more(P<0.05),and the content of K+ and the expression of occludin were significantly lower(P<0.05)in the ipsilateral hemisphere of the model group. Compared with model group, contents of water and Na+ were significantly lower(P<0.05), the GFAP-positive cells were significantly less (P<0.05), and the content of K+ and the expression of occludin were significantly higher(P<0.05) in the ipsilateral hemisphere of sodium aescinate group. Conclusions Sodium aescinate may lessen and (or) delay the genesis of brain edema induced by intracerebral hemorrhage, and improve the prognosis.