自体骨用于脊柱后路植骨融合中BMP-2、BMP-7的表达

目的:观察自体骨用于脊柱后路融合过程中骨形态发生蛋白2-7(BMP-2、BMP-7)的表达,并探讨其在脊柱融合过程中的作用机制。方法:成年雄性新西兰大白兔36只,随机分为2组,每组18只,在兔腰椎5、6横突植入自体骨(自体骨组)、异体骨(异体骨组)制作腰椎后路融合模型。分别于第2、4、7、14、27、35天处死兔,取其融合节段标本,显微镜下观察,并用图文分析软件计算软骨生成面积和BMP-2、BMP-7阳性细胞数。结果:在脊柱后路融合模型中自体骨组、异体骨组软骨生成面积分别为(51±4)%、(40±5)%,自体骨组明显大于异体骨组(P<0.05)。脊柱融合过程中,各时间点自体骨组BMP-2、BMP-7表达均高于异体骨组,差异有统计学意义(P<0.05)。第14~27天表达达到高峰。结论:在脊柱后路植骨融合中,自体骨植骨融合质量优于异体骨,在脊柱融合异位成骨的过程中,自体骨BMP-2、BMP-7起到关键性作用。

BMP-2 and BMP-7 gene expression in spinal posterolateral fusion of autograft

Objective:To investigate the role of bone morphogenetic protein (BMP) in the autograft and allograft spinal fusion. Methods: Thrty-six adult New Zealand rabbits were randomly divided into allograft group and autograft group. The rabbits were undergone L5-L6 intertransvers process fusion using autograft and allograft. The rabbits sacrificed at the 2th, 4th, 7th, 14th, 27th, 35th day after operation, and then tested using immunohistochemical observation and histological observation. Results: The process of spinal fusion, autograft group showed significantly more new bone formation than allograft group (P<0.05). Expressions of BMP-2 and BMP-7 in autograft group were significantly superior to allograft group. Conclusion: The degree of fusion in the posterolateral lumbar, autograft were superior to allograft. BMP-2 and BMP-7 play an important role in the spinal fusion.