前列腺癌组织中血管内皮生长因子的表达及其与内皮素-1的相关性研究

目的:探讨血管内皮生长因子(VEGF)在BPH及PCa中的表达和临床意义,及其与内皮素-1(ET-1)表达的关系。方法:应用免疫组织化学ElivisionTMplus法对44例前列腺癌和36例前列腺增生组织中VEGF和ET-1分别进行免疫组化染色,并在光镜下判断染色部位和染色强度。结果:VEGF在BPH和PCa组织中阳性表达率分别为69.4%和80.9%;阳染部位主要位于肿瘤和增生的腺上皮细胞,间质血管内皮细胞均强阳性;比较BPH和PCa的VEGF表达强度,两者有显著性差异(P<0.05);低分化PCa的ET-1表达强度显著高于中、低分化PCa(P<0.01);在骨转移(BM)PCa中VEGF表达强度显著高于非骨转移(non-BM)癌(P<0.01)。VEGF与ET-1的表达有显著的正相关性(rs=0.780,P<0.01)。结论:VEGF与PCa的发生发展有关,其过度表达可能在PCa的骨转移中起重要作用。VEGF与ET-1可能共同参与了PCa的发生发展。

Expression of VEGF in Prostate Cancer and Its Correlation with ET-1

Objective： To investigate the expressions of VEGF in prostate cancer （PCa） and benign prostatic hyperplasia （BPH）, their clinical significance and their relationship with that of ET-1. Methods ： A total of 44 specimens of PCa and 36 of BPH tissues were examined by the immunohistochemieal ElivisionTM plus method for the expressions of VEGF and ET-1. The intensity of staining for VEGF and ET-1 was assessed by light microscopy on a scale from ＂-＂ to ＂ ＋ ＋ ＋ ＂ Results： The rates of positive expression of VEGF were 69.4% in BPH and 80.9% in PCa, positive staining mostly in the cytoplasm of glandular epithelia and cancer cells, and strongly positive in all the stroma vascular endothelial cells. The staining intensity of VEGF was significantly higher in the PCa than in the BPH group （P 〈 0.05 ）, in the bone metastasis （BM） than in the non-BM group （P 〈 0, 01 ）, and in the lowly than in the highly and moderately differentiated PCa tissues （ P 〈 0.01 ）. The expression of VEGF was positively correlated with that of ET-1 （ rs = 0. 780, P 〈0.01 ）. Conclusion： VEGF is involved in the development, progression and metastasis of PCa. VEGF and ET-1 may play a joint role in its development and progression.