补肾活血通窍中药复方对庆大霉素耳中毒小鼠耳蜗NT-3表达的影响

目的观察补肾活血通窍中药复方对庆大霉素（gentamicin，GM）耳中毒小鼠听性脑干反应（ABR）反应阈及耳蜗神经营养因子-3（neurotrophic factor3，NT-3）表达的影响。方法健康昆明小鼠40只，随机分为5组，每组8只（16耳）。空白对照组正常喂养，GM＋中药组（补肾活血通窍中药复方分为高、中、低三个浓度组）及GM4-生理盐水组每天腹腔注射GM100mg／kg，连续15天，腹腔注射GM第一天起GM＋中药各组分别灌服高、中、低浓度中药复方汤剂，GM＋生理盐水组灌服等量生理盐水，连续20天，灌胃结束后测试ABR反应阈，采用蛋白免疫印迹（Western Blot）方法检测各组耳蜗组织中NT-3的表达。结果与空白对照组比较，GM＋生理盐水组及GM＋低浓度中药组ABR波Ⅲ反应阈显著升高（P〈0．01），耳蜗NT-3蛋白含量下降（P〈0．01）；GM＋高、中浓度中药组ABR波Ⅲ反应阈升高，耳蜗NT-3蛋白含量下降，但差异均无统计学意义（P〉0．05）。结论高、中浓度补肾活血通窍中药复方可显著减轻GM所致的小鼠耳蜗NT-3含量降低和ABR反应阈升高的程度（P〈0．01），从而有效减轻GM对小鼠的耳毒性。

The Effect of Herbal Compound for Reinforcing Kidney,Activating Blood and Arousing Consciousness on the Expression of NT-3 of the Cochlea in Gentamicin-induced Ototoxic Mice

Objective To observe the effects of herbal compound in reinforcing the kidney for activating blood and arousing consciousness(HCRAA) on the ABR threshold and the expression of neurotrophic factor 3(NT-3) of the cochlea of gentamicin(GM)-induced ototoxic mice.Methods 40 mice were randomly divided into normal group,model group,and high,middle and low concentration HCRAA groups.Normal group received no treatment.The model group and the HCRAA groups were intraperitoneally injected with GM 100mg/kg per day for c...