用均匀设计法优化蛇葡萄素的提取与纯化工艺

目的:优选蛇葡萄素的提取工艺。方法:采用均匀设计法安排实验,以蛇葡萄素得率及纯度为评价指标,考察乙醇浓度,回流次数及时间对蛇葡萄素提取率的影响,同时考察石油醚萃取次数,活性炭用量及重结晶次数对蛇葡萄素纯化程度的影响,优选出合理的提取工艺。结果:提取优化条件:乙醇浓度90%,回流4次,每次1.5h;纯化优化条件:石油醚萃取3次,每100 g原药材加活性炭1 g,重结晶3次。结论:本工艺稳定可行,提取率高,可作为提取蛇葡萄素生产工艺的参考。

Optimizing the Extracting Technique of Ampelopsin from Ampelopsis cantoniensis Planch by A Unifrom Design Method

Objective: To optimize the preparation of ampelopsin from Ampelopsis Cantoniensis Planch.Methods: The extraction and purification process was studied by the uniform design with the extract of ampelopsin content and purity as markers. The facters which influence the extraction and the purification of ampelopsin content were studied by uniform design.Results: The optimum extraction and purification process: the concentration for alcohol was 90%,and refluxing quartic,1.5 h each time;extraction by petroleum ether quintic,the mount of active carbon was 1 g/100 g of the medicine material,and recrystaling thrice.Conclusion: This extraction process has higher yield of ampelopsin and is available for production.