肝细胞生长因子对骨髓移植小鼠造血重建的促进作用

目的 观察肝细胞生长因子（HGF）对骨髓移植小鼠造血重建的促进作用。方法 用BALB／c小鼠建立同基因骨髓移植模型，随机将BALB／c小鼠分A、B两组，B组移植后叶7d皮下注射HGF，A组皮下注射PBS，移植后1、7、14、21和28d检测小鼠血红蛋白、白细胞、血小板、骨髓单个核细胞及骨髓造血细胞面积。结果 B组血红蛋白在移植后7、14d与同期A组无差异（P〉0．05），B组血红蛋白在移植后21d高于同期A组（P〈0．05）；B组血小板在移植后7、14及21d高于同期A组（P值分别小于0．01、0．01和0．05）：B组白细胞在移植后7、14、21及28d高于同期A组（P值分别小于0．01、0．01、0．05及0．05）；B组骨髓单个核细胞及骨髓造血细胞面积在移植后7、14、21和28d高于同期A组（P值分别小于0．05、0．01、0．01及0．05）。结论 HGF对骨髓移植小鼠造血重建有一定的促进作用。

Effect of hepatocyie growth factor on haematopoietic reconstruction after syngeneic bone marrow transplantation in mice

[Objective] To observe the effect of hepatocyte growth factor(HGF)on haematopoietic reconstruction after syngeneic bone marrow transplantation in mice.[Methods] The models of syngeneic bone marrow transplantation(BMT)were established in BALB/c mice.BALB/c mice were divided into group A and B,group B were injected subcutaneously with HGF and group A with PBS from day 0 to 7 after BMT.Hemoglobin,white blood cell,blood platelet,bone marrow mono-nucleus cell and the areas of hemopoietic cell were measured on day 1,7,14,21,28.[Results] There were no difference between the hemoglobin of group B and the hemoglobin of group A(P >0.05),The hemoglobin of group B were higher than that of group A on day 21 after BMT(P <0.05);the blood platelet of group B were higher than that of group A on day 7,14,21 after BMT(P value less than 0.01,0.01,0.05 respectively);the white blood cell of group B were higher than that of group A on day 7,14,21,28 after BMT(p value less than 0.01,0.01,0.05,0.05,respectively);the bone marrow mono-nucleus cell and the areas of hemopoietic cell of group B were higher than that of group A on day 7,14,21,28 after BMT(P value less than 0.01,0.01,0.05,0.05,respectively).[Conclusion] HGF can promote haematopoietic reconstruction of mice after BMT at a certain extent.