C-jun,HSP70在大鼠脊髓损伤组织中的表达及甲基强的松龙的干预作用

目的:探讨C-jun, HSP70在大鼠脊髓损伤组织中的表达,并观察甲基强的松龙(MP)的干预作用. 方法:采用Allen's打击法建立急性脊髓损伤(ASCI)模型,观察MP组和NS组在伤后1, 3, 6 h, 1, 2, 3 d时间点组织中C-jun, HSP70阳性反应物的表达. 结果:MP组与NS组脊髓损伤组织中存在C-jun, HSP70的阳性表达. ASCI后C-jun表达增加,3 h最为显著,MP组在伤后1, 3, 6 h时间点C-jun的表达明显少于NS组(P＜0.05). ASCI后HSP70表达增加,1 d最为显著,MP组在伤后6 h, 1, 2 d时间点HSP70的表达明显多于NS组(P＜0.05). C-jun, HSP70在对照组有少量表达. 结论:急性脊髓损伤组织中存在C-jun, HSP70的动态阳性表达,MP可以抑制损伤性因素(C-jun蛋白)的表达,促进保护性因素(HSP70蛋白)的表达,从而减轻继发性脊髓损伤.

Expressions of C-jun and HSP70 in injured spinal cord tissues of rats and interference effects of methylprednisolone

AIM: To discuss the expressions of C-jun and heat shock protein 70 (HSP70) in injured spinal cord tissues of rats and the interference effects of methylprednisolone (MP). METHODS: Fourty-eight female Wistar rats were randomly assigned to MP and normal saline (NS) groups. The acute spinal cord injury models were made by Allen's method.We observed the expressions of C-jun and HSP70 in each group 1, 3, 6 h, 1, 2 and 3 d after injury. RESULTS: Positive expressions of C-jun and HSP70 were found in the injured spinal cord tissues of NS and MP groups. The expression of C-jun increased after acute spinal cord injury, reached the top at 3 h. The expression of C-jun in MP group 1, 3, and 6 h after injury was lower than that in NS group with statistically significant difference (P<0.05). There was no obvious difference between the 2 groups 1, 2 and 3 d after injury. The expression of HSP70 increased after acute spinal cord injury, and get to the maximum at 1 d. The expression of HSP70 in MP group 6 h, 1, and 2 d after injury was higher than that in NS group with statistically significant difference (P<0.05). There was no obvious difference between the 2 groups 1, 3 h and 3 d after injury. There was fewer expression in control groups. CONCLUSION: Positive expressions of C-jun and HSP70 were found in the injured spinal cord tissues. MP can restrain the expression of risk factor (C-jun protein), and promote the expression of protective factor (HSP70). Therefore, MP can relieve secondary spinal cord injury.