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Ethylene bisdithiocarbamate pesticides Maneb and Mancozeb cause metal overload in human colon cells |
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| Institution: | 1. Laboratory of Enzyme engineering and Microbiology, National Engineering School in Sfax, University of Sfax, B.P. 1173, 3038 Sfax, Tunisia;2. Laboratory of Microorganisms and Biomolecules of the Center of Biotechnolgy of Sfax, Sfax, Tunisia;3. Unit of Biotechnology and Pathology, Higher Institute of Biotechnology of Sfax, P.O. Box 261, 3038 Sfax, Tunisia;4. Laboratory of Hematology, CHU Habib Bourguiba, University of Sfax, 3029 Sfax, Tunisia;5. Laboratory of Anatomopathology, CHU Habib Bourguiba, University of Sfax, 3029 Sfax, Tunisia;6. Laboratory of Biochemistry, CHU Hedi Chaker, University of Sfax, 3029 Sfax, Tunisia;1. Dept. of Biotechnological and Applied Clinical Sciences, University of L''Aquila, Via Vetoio, 67100 L''Aquila, Italy;2. Dept. of Life, Health and Environmental Sciences, University of L''Aquila, Via Vetoio, 67100 L''Aquila, Italy |
| Abstract: | Previous studies in our laboratory have shown that ethylene bisdithiocarbamate (EBDC) fungicides Maneb and Mancozeb are equipotent gastrointestinal toxicants that produce cell loss and metal accumulation within HT-29 and Caco2 colon cells. Nabam, MnCl2, CuCl2 and ZnCl2 exposure produced no loss of viability up to 200 μM and increases in metal levels were noted but not to the same extent as Maneb and Mancozeb. EBDC exposure caused increases in copper levels (20–200 μM). Maneb and Mancozeb treatment also caused increases in manganese and zinc concentrations (20–200 μM). Nabam plus MnCl2 and Nabam and MnCl2 plus ZnCl2 caused decreases in viability and increases in metal levels comparable to Maneb and Mancozeb. Decreases in the ratio of reduced glutathione to glutathione disulfide were observed with Maneb and Mancozeb (20–200 μM). Maneb and Mancozeb treatment results in intracellular metal accumulation leading to the oxidative stress. The metal moiety and the organic portion of EBDCs contribute to toxicity. |
| Keywords: | Maneb Mancozeb Zineb Colon cells Metal accumulation Fungicides copper chloride DTCs"} {"#name":"keyword" "$":{"id":"kw0050"} "$$":[{"#name":"text" "_":"dithiocarbamates EBDC"} {"#name":"keyword" "$":{"id":"kw0060"} "$$":[{"#name":"text" "_":"ethylene bisdithiocarbamate GI"} {"#name":"keyword" "$":{"id":"kw0070"} "$$":[{"#name":"text" "_":"gastrointestinal ICP-OES"} {"#name":"keyword" "$":{"id":"kw0080"} "$$":[{"#name":"text" "_":"inductively coupled plasma-optical emission spectroscopy MZ"} {"#name":"keyword" "$":{"id":"kw0090"} "$$":[{"#name":"text" "_":"Mancozeb MB"} {"#name":"keyword" "$":{"id":"kw0100"} "$$":[{"#name":"text" "_":"Maneb manganese chloride MTT"} {"#name":"keyword" "$":{"id":"kw0120"} "$$":[{"#name":"text" "_":"3-(4 5-dimethylthiazol-2-yl)-2 5-diphenyltetrazolium bromide NB"} {"#name":"keyword" "$":{"id":"kw0130"} "$$":[{"#name":"text" "_":"Nabam ROS"} {"#name":"keyword" "$":{"id":"kw0140"} "$$":[{"#name":"text" "_":"reactive oxygen species TBARS"} {"#name":"keyword" "$":{"id":"kw0150"} "$$":[{"#name":"text" "_":"thiobarbituric acid reactive substances zinc chloride ZB"} {"#name":"keyword" "$":{"id":"kw0170"} "$$":[{"#name":"text" "_":"Zineb |
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