Effect of active fraction of Eriocaulon sieboldianum on human leukemia K562 cells via proliferation inhibition,cell cycle arrest and apoptosis induction |
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| Affiliation: | 1. Department of Pharmacy, General Hospital of Shenyang Military Area Command, Shenyang 110840, China;2. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China;1. Vinča Institute of Nuclear Sciences, University of Belgrade, P.O. Box 522, 11000 Belgrade, Serbia;2. IChTM – Department of Catalysis and Chemical Engineering, University of Belgrade, Njegoševa 12, 11000 Belgrade, Serbia;3. Faculty of Physical Chemistry, University of Belgrade, Studentski trg 12-16, P.O.Box 47, 11158 Belgrade, Serbia;1. Hematology Department, Hospital del Mar, Barcelona, Spain;2. Pathology Department, Hospital del Mar, Barcelona, Spain;3. Universitat Autònoma de Barcelona, Barcelona, Spain;4. Universitat Pompeu Fabra, Barcelona, Spain;5. Grup de Recerca Clínica Aplicada en Neoplàsies Hematològiques-Hospital del Mar-IMIM, Barcelona, Spain;1. Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo (HC FMUSP), São Paulo, SP, Brazil;2. Instituto Hemomed, São Paulo, SP, Brazil |
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| Abstract: | Eriocaulon sieboldianum (Sieb. & Zucc. ex Steud.), a genus of Eriocaulon in the Eriocaulaceae family, is an edible and medicinal plant used in traditional Chinese medicine. It was processed into healthcare beverages for expelling wind-heat, protecting eyes, and reducing blood fat. Also, it has been used with other herbs as Traditional Chinese herbal compound to treat cancer as adjuvants in tumor therapy in China. However, the active fractions and precise cellular mechanisms of E. sieboldianum extract remain to be illustrated. The goal of this study was to investigate the effects of the active fraction of E. sieboldianum on the growth of K562 cells and understand the possible mechanisms of its action. Our findings suggested that the fraction E3 of E. sieboldianum could effectively inhibit the activity of Aurora kinase and induce apoptosis via blocking cell cycle, up-regulating the expression of proapoptotic proteins including p53 and Bax and reducing the expression of Bcl-2. The levels of Cytochrome C, cleaved caspase-9, cleaved caspase-3 and cleaved PARP were also found to be increased after treatment with fraction E3 of E. sieboldianum.This study could improve the development of E. sieboldianum and raise its application value in cancer adjuvant therapy. Considering it is both a dietary supplement and a traditional Chinese herbal medicine which exhibits anticancer activities, it can be developed into functional food. |
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| Keywords: | Aurora kinase Cell cycle arrest Apoptosis K562 cells |
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