HPLC法测定阿咖酚胶囊中对乙酰氨基酚、阿司匹林和咖啡因的含量

目的建立HPLC法测定阿咖盼胶囊中三种成分的含量。方法 采用Phenomenex C18色谱柱，以甲醇-1％醋酸溶液（65：35）（用三乙胺调节pH至3．5）为流动相．柱温为室温，流速为1．0mL·min^-1,检测波长272nm。结果对乙酰氨基酚在1．263μg～6．316μg范围内与相应峰面积呈良好线性关系（r=0．9994），平均回收率为100．45％，RSD为0．95％；阿司匹林在2．305μg～11．525μg范围内与相应峰面积呈良好线性关系（r=0．9990），平均回收率为99．20％，RSD为1．2％；咖啡因在0．302pg～1．510μg范围内与相应峰面积呈良好线性关系（r=0．9999），平均回收率为101．20％．RSD为0．8％。结论方法简便，结果准确．可作为本品质量和含量测定控制方法。

Determination of paracetamol,caffeine and aspirin in Aka-fen capsules by HPLC

OBJECTIVE A HPLC method for the content determination of paracetamol, caffeine and aspirin in Aka-fen capsules by HPLC was established. METHODS The determination was performed on Phenomenex C18 column with the mobile phase consisted of methanol-1% acetic acid solution （65：35）（pH was adjusted to 3.5 with triethylamine） at ambient temperature.The flow rate was 1.0mL·min^-1.The detection wavelength was 272nm. RESULTS The paracetamol curve was linear in the range from 1.263ttg to 6. 316μg（r = 0. 9994）and the average recovery was 100.45 % （ RSD = 0.95 96 ） . The aspirin curve was linear in the range from 2. 305μg to 11. 525μg（r = 0. 9990）and the average recovery was 99.20 % （RSD = 1.2 % ）. The caffeine curve was linear in the range from 0.302μg to 1.510μg （r = 0.9999）and the average recovery was 101.20% （RSD = 0.8%） . CONCLUSION The methods is simple and reliable. It is suitable for the content control of Aka-fen capsules.