利用噬菌体随机肽库筛选表达Aβ靶向肽的噬菌体

目的抗Aβ神经毒性的Aβ靶向肽噬菌体的筛选。方法分别合成Aβ1-10序列组成的短肽并将其生物素化;以生物素化Aβ1-10为靶分子,用噬菌体展示技术及液相亲和捕获法筛选出特异性高亲和力的噬菌体;采用生物传感分析技术,通过结合特异性实验和短肽竞争性抑制实验,检测所筛选的噬菌体与靶分子之间的亲和动力学关系。结果经过对靶分子Aβ1-10的3轮筛选,筛出了与Aβ1-10特异性结合的单克隆噬菌体;生物传感分析技术结果进一步表明,靶分子（Aβ1-10）与所筛选噬菌体之间的结合具有高度特异性。结论筛选出了展示特异性高亲和力结合Aβ1-10短肽的噬菌体。

Identification of phages expressed β-amyloid-targeting peptide using phage display random peptide library

Objective To screen the targeting peptides of &;#61538;-amyloid (A&;#61538;) for attenuation of the neural toxicity of Aβ. Methods Aβ1-10 was synthesized and labeled by biotin. A randomized 12-mer peptide library presented on M13 phages was used to screen the peptides (phages) specifically bond to the bio-Aβ1-10. The phages specifically binding to the bio-Aβ1-10 was examined by BIAcore3000 instrument. The affinity and kinetics of the interaction between the screened phages and the target molecule were detected by binding specificity test of the screened phages and competitive inhibition test of the small peptides using bio-sensing techniques. Results The phages with binding affinity for bio-Aβ1-10 were acquired after 3-round screen. Bio-sensing technique showed high binding specificity between the selected phages and bio-Aβ1-10. Conclusion The phages with high affinity and binding specificity with Aβ1-10 can be obtained by using bio-sensing techniques.