百两金根的化学成分研究

目的 百两金为传统苗药,其化学成分的有关研究报道较少,本研究目的在于研究百两金的化学成分。方法 将百两金干燥根体积分数70%乙醇提取物利用硅胶、重结晶、ODS及制备/半制备HPLC进行分离纯化,根据理化性质及波谱数据对所得化合物结构进行鉴定。结果 从百两金根中得到了20个化合物。分别为岩白菜素 (1)、甲基岩白菜素 (2)、11-O-没食子酰岩白菜素 (3)、 syringin (4)、(+)-syringaresinol-O-β-D-glucopyranoside (5)、(-)-(7R,8S,7′R,8′S)-4,9,4′,9′-tetrahydroxy-3,3′-dimethoxy-7,7′-epoxylignan-9-O-β-D-xylopyranoside (6)、saracoside (7)、isolariciresinol-4-O-β-D-glucopyranoside (8)、(7S,8R)-urolignoside (9)、staphylionoside D (10)、蚱蜢酮 (11)、l-borneol 6-O-β-D-aiosyl-β-D-glucoside (12)、(+)-angelicoidenol 2-O-β-D-glucopyranoside (13)、牡荆素-2″-O-鼠李糖苷 (14)、根皮苷 (15)、phenethyl alcohol β-D-(2′-O-β-D-glucopyranosyl)glucopyranoside (16)、phenylethyl-β-D-glucopyranoside (17)、菖蒲碱C (18)、己内酰胺 (19)、蔗糖 (20)。结论 其中化合物5~19为首次从紫金牛科植物中分离得到,化合物4为首次从紫金牛属植物中分离得到,化合物2和3为首次从该植物中分离得到。其中化合物6是自国内学者2013年从岭南杜鹃分离得到以来第二次被分离得到。

Chemical Constituents from the Roots of Ardisia crispa (Thunb.) A. DC.

OBJECTIVE To enrich the chemical constituents of Ardisia crispa(Thunb.) A. DC.. METHODS The 70% ethanol extract of Ardisia crispa (Thunb.) A. DC. was isolated and purified by silica, recrystallization, ODS and preparative / semi-preparative HPLC, and the structures of obtained compounds were identified by physico-chemical properties and spectroscopic data. RESULTS The compounds are bergenin (1), methylbergenin (2), 11-O-galloylbergenin (3), syringin (4), (+)-syringaresinol-O-β-D-glucopyranoside (5), (-)-(7R, 8S, 7′R, 8′S)-4, 9, 4′, 9′-tetrahydroxy-3, 3′-dimethoxy-7, 7′-epoxylignan-9-O-β-D-xylopyranoside (6), saracoside (7), isolariciresinol-4-O-β-D-glucopyranoside (8), (7S, 8R)-urolignoside (9), staphylionoside D (10), grasshopper ketone (11), l-borneol 6-O-β-D-aiosyl-β-D-glucoside (12), (+)-angelicoidenol-2-O-β-D-glucopyranoside (13), vitexin-2″-O-rhamnoside (14), phlorizin (15), phenethyl alcohol-β-D-(2′-O-β-D-glucopyranosyl) glucopyranoside (16), phenylethyl-β-D-glucopyranoside (17), tatarine C (18), caprolactam (19) and sucrose (20). CONCULUSION Compounds 5-19 are firstly isolated from the family Myrsinaceae, compound 4 is isolated from genus Ardisia for the first time and compound 2 and 3 are obtained from this plant for the first time. Compound 6 is the second occurrence ever domestic scholars obtained it from Rhododendron mariae Hance in 2013.