The expression and role of protein kinase C (PKC) epsilon in clear cell renal cell carcinoma |
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| Authors: | Bin Huang Kaiyuan Cao Xiubo Li Shengjie Guo Xiaopeng Mao Zhu Wang Jintao Zhuang Jincheng Pan Chengqiang Mo Junxing Chen Shaopeng Qiu |
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| Affiliation: | 1.Department of Urology, the First Affiliated Hospital, Sun Yat-Sen University, Guangzhou (510080), China;2.Research Center for Clinical Laboratory Standard, Zhongshan Medical School, Sun Yat-sen University, Guangzhou (510080), China;3.Pulmonary disease institute, Guangzhou Chest Hospital Pulmonary Disease Institute, Guangzhou (510095), China;4.Department of Urology, Sun Yat-Sen University Cancer Center, Guangzhou (510060), China |
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| Abstract: | Protein kinase C epsilon (PKCε), an oncogene overexpressed in several human cancers, is involved in cell proliferation, migration, invasion, and survival. However, its roles in clear cell renal cell carcinoma (RCC) are unclear. This study aimed to investigate the functions of PKCε in RCC, especially in clear cell RCC, to determine the possibility of using it as a therapeutic target. By immunohistochemistry, we found that the expression of PKCε was up-regulated in RCCs and was associated with tumor Fuhrman grade and T stage in clear cell RCCs. Clone formation, wound healing, and Borden assays showed that down-regulating PKCε by RNA interference resulted in inhibition of the growth, migration, and invasion of clear cell RCC cell line 769P and, more importantly, sensitized cells to chemotherapeutic drugs as indicated by enhanced activity of caspase-3 in PKCε siRNA-transfected cells. These results indicate that the overexpression of PKCε is associated with an aggressive phenotype of clear cell RCC and may be a potential therapeutic target for this disease. |
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| Keywords: | Protein kinase C epsilon Renal cell carcinoma Clear cell |
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