蒙药材黄花铁线莲抗肿瘤活性成分研究

目的:研究黄花铁线莲Clematis intricata干燥带花枝条中的抗肿瘤活性成分。方法:采用海虾幼虫致死实验为抗肿瘤活性追踪测试模型,硅胶、SephadexLH-20和制备型HPLC等色谱手段进行分离纯化,根据波谱数据结合理化性质鉴定化合物结构。结果:分离鉴定了7个黄酮,分别为芹菜素(1)、木犀草素-3’-O-β-D-吡喃葡萄糖苷(2)、槲皮素-3-O-β-D-吡喃葡萄糖苷(3)、芦丁(4)、山奈酚-3-O-α-L-吡喃鼠李糖基(1→6)-β-D-吡喃葡萄糖苷(5)、芹菜素-7-O-(6″-E-p-香豆酰基)-β-D-葡萄糖苷(6)、芹菜素-6-C-(6″-E-p-香豆酰基)-β-D-葡萄糖苷(7)。结论:7个化合物均为首次从该植物中分离得到。

Study on the Antitumor Constituents from a Traditional Mongolian MedicalHerb Clematis intricata Bunge

Objective:To investigate the potential anti-tumor compounds derived from the dried flowering branch of Clematis intricata.Methods:The study was carried out by brine shrimp lethality bioassayguided fractionation,and repeated chromatography with silicagel,Sephadex LH-20 and pre-HPLC.The compounds obtained were identified on the basis of their physicochemical and spectroscopic evidences.Results:Sevencompoundswereisolatedandtheirstructureswereelucida-tedasapigenin(1),luteolin-3′-O-β-D-glucopyranoside(2),quercetin-3-O-β-D-glucopyranoside(3),rutin(4),kaempferol-3-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside(5),Apigenin-7-O-(6″-E-p-coum-aroyl)-β-D-glucopyranoside(6),Apigenin-6-C-(6″-E-p-coumaroyl)-β-D-glucopyrano side(7).Conclusion:All these compounds were isolated from the plant for the first time.