Preparative Separation of Four Alkaloids from Gelsemium elegans by High-speed Counter-current Chromatography |
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| Authors: | Qiu-ping Zhang Zheng-tao Wang Gui-xin Chou |
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| Affiliation: | Key Laboratory of Standardization of Chinese Medicines, Ministry of Education, Institute of Chinese Materia Medica of Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Key Laboratory of General Administration of Sport, Shanghai Research Institute of Sports Science, Shanghai 200030, China;Shanghai R&D Center for Standardization of Chinese Medicines, Shanghai 201203, China;Key Laboratory of Standardization of Chinese Medicines, Ministry of Education, Institute of Chinese Materia Medica of Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Shanghai R&D Center for Standardization of Chinese Medicines, Shanghai 201203, China;Key Laboratory of Standardization of Chinese Medicines, Ministry of Education, Institute of Chinese Materia Medica of Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Shanghai R&D Center for Standardization of Chinese Medicines, Shanghai 201203, China |
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| Abstract: | Objective To develop an efficient preparative method for the separation of Gelsemium alkaloids from Gelsemium elegans. Methods High-speed counter-current chromatography (HSCCC) with several two-phase solvent systems was investigated for the separation of Gelsemium alkaloids. The purity and structure identification of the purified compounds were performed with HPLC and NMR spectra, respectively. Results In a single operation, 206.6 mg of crude alkaloid sample was separated to yield 28.7 mg of koumine, 24.9 mg of gelsemine, 26.9 mg of humantenine, and 7.2 mg of gelsevirine, with the purities of 97.8%, 95.4%, 97.4%, and 93.5%, respectively. Conclusion A preparative HSCCC method is successfully established for the separation of four Gelsemium alkaloids from G. elegans with a modified two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-0.5% triethylamine-H2O (3:5:3:4). |
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| Keywords: | high-speed counter-current chromatography Gelsemium alkaloids Gelsemium elegans separation |
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