神经生长因子转染的人脐带间充质干细胞促进中枢神经系统损伤修复的实验研究

目的 探讨神经生长因子（NGF）转染的人脐带间充质干细胞（hUC-MSCs）减轻中枢神经系统功能损伤和促进中枢神经系统功能恢复的可行性。方法 构建缺血、缺氧脑损伤的Wistar 大鼠模型，同时分离培养hUC-MSCs，并将腺病毒介导的NGF转染hUC-MSCs。将转染成功后的hUC-MSCs移植入大鼠模型中，饲养4 周后采用足印分析试验、平衡木试验分别对大鼠的运动功能进行评价。结果 流式细胞仪检测提示hUC-MSCs 分离培养成功，hUC-MSCs 可过表达NGF 蛋白。与hUC-MSCs 组比较，NGF-hUC-MSCs 组幼鼠右侧足印步态重复间距和平衡木通过时间均有统计学差异（均P＜0.05），左侧足印步态重复间距无统计学差异（P＞0.05）。结论 NGF 联合hUC-MSCs 移植能促进实验大鼠的运动功能恢复，为中枢神经系统损伤修复和并发症预防提供了新的途径。

Human umbilical cord mesenchymal stem cells transfected with nerve growth factor promote injury recovery of central nerves in rats

Objective To investigate the effect of human umbilical cord mesenchymal stem cells (hUC-MSCs) transfected with nerve growth factor (NGF) on injury recovery of central nerves in rats. Methods The isolated and cultured hUC-MSCs were transfected with adenovirus-mediated NGF gene. Ischemia hypoxia model of central nervous system was induced in Wistar rats. The NGF-transfected hUC-MSCs were transplanted in the model rats. The motion function of rats was evaluated with footprint test and balance beam test. Results The transfected hUC-MSCs successfully expressed NGF protein. The footprint test and balance beamtest showed that NGF-transfected hUC-MSCs promoted motor function recovery in rats with ischemic injury of central nerves. Conclusion NGF-transfected hUC-MSCs can improve damage repair of central nerve in rats.