ι-四氢巴马汀抑制羟考酮引起小鼠行为敏化的实验研究

目的探讨ι-四氢巴马汀(-tetrahydropalmatine,ι-THP)对羟考酮(oxycodone,oxy)行为敏化的影响和作用机制。方法采用连续7d给小鼠注射羟考酮(5mg.kg-1,ip),停药5d,d13用相同剂量的羟考酮(5mg.kg-1,ip)激发致小鼠行为敏化模型,利用红外光束自主活动测定仪测定小鼠自发活动的实验方法。结果ι-THP(6.25,12.5,18.75mg.kg-1,ig)一次或多次给药,对小鼠的自发活动没有影响,但ι-THP(18.75mg.kg-1,ig)能抑制羟考酮(5mg.kg-1,ip)引起的小鼠行为敏化的形成和表达。阿朴吗啡(0.5、1.0mg.kg-1,ip)给药后50min自身不影响小鼠的自发活动,但阿朴吗啡(1.0mg.kg-1,ip)能翻转ι-THP(18.75mg.kg-1,ig)抑制羟考酮小鼠行为敏化形成的作用,但不影响ι-THP抑制羟考酮行为敏化表达的作用。结论ι-THP抑制羟考酮引起的小鼠行为敏化的形成和表达,ι-THP抑制羟考酮行为敏化的形成可能与抑制DA受体相关,而抑制表达可能与抑制DA受体无关。

Studies on effects of ι-tetrahydropalmatine on locomotor sensitivity induced by oxycodone in mice

Aim To investigate effects of ι-tetrahydropalmatine on locomotor sensitivity induced by oxycodone in mice and its mechanisms.Mothods Mice were administered for 7consecutive days with oxycodone(5 mg·kg-1,ip).After 5 d withdrawal period,all animals were injected with oxycodone(5 mg·kg-1)and then put into the test chambers to record their locomotor activities for 60 min.Results ι-THP did not affect the locomotor activity per set,but ι-THP(18.75 mg·kg-1,ig)inhibited the development and expression of locomotor sensitivity induced by oxycodone in mice;apomorphine(0.5,1.0 mg·kg-1,ip)did not affect the locomotor activity per set,but apomorphine could sheerly inhibited the effects of ι-THP on the development of the locomotor sensitivity by oxycodone,and there were no effects of ι-THP on the expression of the locomotor sensitivity.Conclusions ι-THP inhibited the deveolpment and expression of locomotor sensitivity induced by oxycodone in mice,and the meschanims of ι-THP on the deveolpment of locomotor sensitivity might be correlated with DA receptor,but the meschanims of ι-THP on the sensitivity expression might not be involved.