蛴螬生物碱提取工艺及体内外抗肿瘤活性研究

目的研究蛴螬生物碱的提取工艺并对其抗肿瘤活性进行评价。方法采用单因素实验和L9(34)正交实验得到最佳提取蛴螬生物碱的方法;采用MTT和荷瘤小鼠抑瘤率的方法测定生物碱的体内外抗肿瘤活性。结果最佳提取工艺为蛴螬粗粉用体积分数95%乙醇浸泡提取24h;蛴螬生物碱对体内外肿瘤细胞有明显抑制作用,且具有质量浓度依赖性。结论蛴螬粗粉用体积分数95%乙醇浸泡24h提取,浓缩浸膏经过酸化萃取得到的生物碱抗肿瘤活性显著。

Study on the extraction technique of alkaloids from Holotrichia diomphalia Bates and their antitumor activity in vitro and in vivo

Objective To optimize the extracting technique for alkaloids from Holotrichia diomphalia Bates and to evaluate their an-titumor activity .Method Single factor analysis and L9 （34 ） orthogonal test were used to optimize the extraction method of Ho-lotrichia diomphalia Bates alkaloid .The anti-tumor activities of alkaloids in vitro were evaluated in human cancer cells by MTT assay .S180 sarcoma mice were used for the anti-tumor test in vivo .Results The optimal condition was to dip the coarse powder of Holotrichia diomphalia Bates for 24 h in 95% alcohol .The alkaloids from Holotrichia diomphalia Bates inhibited human cancer cells and development of transplanted tumor in mice in a dose dependent manner .Conclusion The optimal condition was to dip the coarse powder of Holotrichia diomphalia Bates for 24 h in 95% alcohol .After filtrating condensing ,and acidification ,the extraction was performed .The alkaloid from Holotrichia diomphalia Bates showed significant anti-tumor activities .