| 高效液相色谱法测定多维元素片中泛酸的含量 |
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| 引用本文： | 那海秋,高广慧,赵 飞,等. 高效液相色谱法测定多维元素片中泛酸的含量[J]. 西北药学杂志, 2014, 0(2): 145-147 |
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| 作者姓名： | 那海秋 高广慧 赵 飞 等 |
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| 作者单位： | 辽宁省食品药品检验所,沈阳110023 |
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| 摘 要： | 目的建立复合多维元素片中泛酸的含量测定方法。方法高效液相色谱法,C18色谱柱(250mm×4.6mm,5μm);流动相:乙腈-1mL·L-1磷酸水溶液(5∶95);流速:1.0mL·min-1;柱温:25℃;检测波长:200nm。结果泛酸质量浓度在1.32132.32μg·mL-1范围内具有良好的线性关系,相关系数r=0.999 9,平均回收率为99.3%,RSD为1.4%(n=12)。结论该方法快速简便,结果准确,适用于多维元素片中泛酸含量测定。
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| 关 键 词： | 泛酸 高效液相色谱法 含量测定 |
Determination of pantothenic acid in Vitamins with Minerals Tablets by HPLC |
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| NA Haiqiu,GAO Guanghui,ZHAO Fei,HUANG Lina. Determination of pantothenic acid in Vitamins with Minerals Tablets by HPLC[J]. Northwest Pharmaceutical Journal, 2014, 0(2): 145-147 |
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| Authors: | NA Haiqiu GAO Guanghui ZHAO Fei HUANG Lina |
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| Affiliation: | (Liaoning Institute for Food and Drug Control, Shenyang 110023, China) |
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| Abstract: | Objective To develop a method for determining the content of pantothenic acid in Vitamins with Minerals Tablets .Meth-ods The HPLC was carried out on a C18 （25 mm ＆#215; 4 .6 mm ,5 μm） column ;the mobile phase consisted of acetonitrile-1 mL ＆#183; L -1 phosphoric acid solution（5∶95）with flow rate of 1 .0 mL ＆#183; min-1 ;the column temperature was set at 25℃ ;the detection wave-length was set at 200 nm .Results The linearity of the method was ranged from 1 .32 to 132 .32 μg ＆#183; mL -1 （r=0 .999 9） ,and the average recovery rate was 99 .3% ,with RSD 1 .4% （n=12） .Conclusions This method is rapid ,simple ,accurate ,and applicable to the determination of pantothenic acid in Vitamins with Minerals Tablets . |
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| Keywords: | pantothenic acid HPLC content determination |
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