Cordyceps pruinosa extracts induce apoptosis of HeLa cells by a caspase dependent pathway |
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Authors: | Ho Gyoung Kim Heesang Song Deok Hyo Yoon Byeong-Wook Song Sang Min Park Gi Ho Sung Jae-Youl Cho Hae Il Park Sunga Choi Won O Song Ki-Chul Hwang Tae Woong Kim |
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Institution: | 1. Department of Biochemistry, Kangwon National University, Chuncheon 200-701, Republic of Korea;2. Research Institute of Science for Aging, Yonsei University, Seoul 120-752, Republic of Korea;3. Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea;4. Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331, USA;5. School of Bioscience & Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea;6. Division of Pharmacology, Kangwon National University, Chuncheon 200-701, Republic of Korea;g Department of Life Science, Hallym University, Chuncheon 200-702, Republic of Korea;h Department of Food Science and Human Nutrition, Michigan State University, East Lansing, MI 48824, USA;i Cardiovascular Research Institute, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea |
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Abstract: | Aim of the studyCordyceps is a parasitic fungus and has long been used as a traditional Chinese medicine to treat illnesses, promote longevity, increase athletic power, and relieve exhaustion and cancer. In this study, we reveal the mechanisms underlying apoptosis induced by Cordyceps pruinosa butanol fraction (CPBF) in the human cervical adenocarcinoma cell line, HeLa.Materials and methodsProliferation and apoptosis of cells were examined by MTT assay, DNA fragmentation, phosphatidyl serine distribution assay, Western blot analysis, and immunocytochemistry. To determine the association between CPBF related apoptosis and ROS, electron spin resonance (ESR) trapping experiments were used.ResultsCPBF inhibited proliferation and induced apoptosis in HeLa cells in a dose-dependent manner using a MTT assay, DNA fragmentation, and a phosphatidyl serine distribution assay. Western blot analysis showed that apoptosis in HeLa cells was caspase-3- and -9-dependent. Proteolytic cleavage of PARP and the release of cytochrome c from the mitochondria into the cytosol were significantly increased and the Bcl-2/Bax protein ratio was decreased. Apoptosis induced by CPBF was not prevented by various antioxidants.ConclusionsThese results indicate that apoptotic effects of CPBF on HeLa cells are mediated by mitochondria-dependent death-signaling pathway independent of reactive oxygen species, suggesting that CPBF might be effective as an anti-proliferative agent for cancer. |
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Keywords: | CPBF Cordyceps pruinosa butanol fraction TUNEL terminal deoxynucleotidyl transferase dUTP nick end labeling PARP poly(ADP-ribose) polymerase ROS reactive oxygen species ESR electron spin resonance |
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