Cytoprotective effect of the fruits of Lycium chinense Miller against oxidative stress-induced hepatotoxicity |
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Authors: | Rui Zhang Kyoung Ah Kang Mei Jing Piao Ki Cheon Kim Areum Daseul Kim Sungwook Chae Jong Sang Park Ui Joung Youn Jin Won Hyun |
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Affiliation: | 1. School of Medicine and Applied Radiological Science Research Institute, Jeju National University, Jeju-si 690-756, Republic of Korea;2. School of Marine Biomedicinal Sciences, Jeju National University, Jeju-si 690-756, Republic of Korea;3. Department of Herbal Resources Research, Korea Institute of Oriental Medicine, Daejeon 305-811, Republic of Korea;4. Haklimsu Research Institute of Life Engineering Inc., Daejeon 302-220, Republic of Korea;5. The Center for Cell Signaling & Drug Discovery Research, College of Pharmacy, Ewha Womans University, Seoul 120-750, Republic of Korea |
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Abstract: | Aim of the studyFruits of Lycium chinense Miller (Solanaceae), distributed in northeast Asia, have gained attraction for their hepatoprotective role in traditional oriental medicine.The excessive production of reactive oxygen species (ROS) is hazardous for living organisms and damage major cellular constituents such as DNA, lipid, and protein. The cytoprotective effect of Lycium chinense fruits (Lycium extract) was assessed against H2O2-induced Chang liver cell damage.Materials and methodsThe effect of Lycium extract against H2O2-induced cell death was determined by the MTT assay. Radical scavenging activity was determined through the assessments of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, intracellular ROS, hydroxyl radicals, and superoxide. The inductions of antioxidant enzymes were determined via their protein expressions and activities. DNA damage was measured using comet assay and expression of phospho-histone H2A.X. Lipid peroxidation was measured using 8-isoprostane level and fluorescent probe. Protein modification was measured using protein carbonyl moiety.Results and conclusionLycium extract scavenged the DPPH free radicals, intracellular ROS, hydroxyl radicals, and superoxide. Lycium extract recovered activities of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) decreased by H2O2. Lycium extract decreased DNA damage, lipid peroxidation and protein carbonyl values increased by H2O2 exposure. In addition, Lycium extract increased the cell viability of Chang liver cells exposed to H2O2 via inhibition of apoptosis. These results show that Lycium extract protected Chang liver cells against oxidative stressed cell damage by H2O2 via scavenging ROS and enhancing antioxidant enzyme activity. |
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Keywords: | Reactive oxygen species Lycium chinense Chang liver cells Antioxidant enzyme DNA damage Lipid peroxidation Protein carbonyl |
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