Insulin releasing and alpha-glucosidase inhibitory activity of ethyl acetate fraction of Acorus calamus in vitro and in vivo |
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Authors: | Mei-mei Si Jian-shu Lou Chang-Xin Zhou Juan-Na Shen Hong-Hai Wu Bo Yang Qiao-Jun He Hao-Shu Wu |
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Institution: | 1. Institute of Pharmacology & Toxicology and Biochemical Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, 388 Yu-hang-tang Road, Hangzhou, China;2. Department of Traditional Chinese Medicine and Natural Drug Research, College of Pharmaceutical Science, Zhejiang University, Hangzhou, China |
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Abstract: | Ethnopharmacological relevanceThe radix of Acorus calamus L. (AC) is widely used in the therapy of diabetes in traditional folk medicine of America and Indonesia, and we previously reported the insulin sensitizing activity of the ethyl acetate fraction of AC (ACE).Aim of the studyTo investigate the insulin releasing and alpha-glucosidase inhibitory activity of ACE in vitro and in vivo.Materials and methodsInsulin releasing and alpha-glucosidase inhibitory effects of different fractions from AC were detected in vitro using HIT-T15 cell line and alpha-glucosidase enzyme. Furthermore, effects of ACE orally on serum glucose were detected in fasted and glucose/amylum challenged normal mice.ResultsAC and ACE increased insulin secretion in HIT-T15 cells as gliclazide did. As in vivo results, ACE (400 and 800 mg/kg) significantly decreased fasting serum glucose, and suppressed the increase of blood glucose levels after 2 g/kg glucose loading in normal mice. In addition, ACE as a mixed-type inhibitor inhibited alpha-glucosidase activity in vitro with an IC50 of 0.41 μg/ml, and 100 mg/kg of it clearly reduced the increase of blood glucose levels after 5 g/kg amylum loading in normal mice.ConclusionsApart from its insulin sensitizing effect, ACE may have hypoglycemic effects via mechanisms of insulin releasing and alpha-glucosidase inhibition, and thus improves postprandial hyperglycemia and cardiovascular complications. |
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Keywords: | AC Acorus calamus L ACB n-butanol fraction of AC ACE ethyl acetate fraction of AC ACP petroleum ether fraction of AC ACW water fraction of AC CMC-Na sodium carboxymethyl cellulose DMSO dimethyl sulfoxide PNPG p-nitrophenyl-β-d-galactopyranoside |
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