In vitro antiprotozoal,antimicrobial and antitumor activity of Pavetta crassipes K. Schum leaf extracts |
| |
| Authors: | Elhadj S. Baldé ,Vé ronique Megalizzi,Mohamed S. Traoré ,Paul Cos,Louis Maes,Christine Decaestecker,Luc Pieters,Aliou M. Baldé |
| |
| Affiliation: | 1. Département de Pharmacie, Faculté de Médecine, Pharmacie et Odontostomatologie, Université de Conakry, BP 6411 Conakry, Guinea;2. Laboratory of Toxicology, Institute of Pharmacy, Université Libre de Bruxelles (ULB), Boulevard du Triomphe, B-1050 Brussels, Belgium;3. Laboratory of Microbiology, Parasitology and Hygiene (LMPH), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, Belgium;4. Laboratory of Pharmacognosy and Pharmaceutical Analysis, Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium |
| |
| Abstract: | Aim of the studyTo study the potential benefit of the traditional medicinal plant Pavetta crassipes K. Schum (Rubiaceae), which is widely distributed throughout West Africa, the methanol and dichloromethane extracts were isolated from the plant leaves to determine if they exhibited antiprotozoal, antibacterial, antifungal or antitumor activity in vitro.Materials and methodsThe methanol and dichloromethane extracts and their specific fractions were obtained using bioassay-guided fractionation and investigated for antiproliferative activity in vitro in microorganisms (Staphylococcus aureus, Escherichia coli and Candida albicans), protozoans (Trypanosoma cruzi, Trypanosoma brucei, Leishmania infantum and Plasmodium falciparum), and cancer (U373, PC3, MXT and A549) and normal cell lines (NHDF and MRC-5).ResultsMost of the alkaloid fractions investigated exhibited antiproliferative activity in all the cancer cell lines, microorganisms and protozoans studied.ConclusionsThe benefit of Pavetta crassipes as a traditional medicinal remedy was confirmed using antiprotozoal and cytotoxicity assays in vitro. These analyses revealed that the components present in the alkaloid extract of Pavetta crassipes are responsible for its antiprotozoal and cytotoxic efficacy. |
| |
| Keywords: | ATCC, American type culture collection EPS, extracellular polymeric substance GBM, glioblastoma HMI, Hirumi-9 medium IC50, 50% inhibitory growth concentration MEM, minimal essential medium MIC, minimal inhibitory concentration MTT, 3-[4,5-dimethylthiazol-2yl]-diphenyl tetrazolium bromide NBT, nitro blue tetrazolium NCI, National Cancer Institute NHDF, normal human dermal fibroblast NSCLC, non-small-cell lung cancer PES, phenazine ethosulfate SAB, Sabouraud SI, selectivity index TSB, tryptic soy broth |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
