Protective effect of 5-hydroxymethylfurfural derived from processed Fructus Corni on human hepatocyte LO2 injured by hydrogen peroxide and its mechanism |
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Authors: | Xia Ding Ming-Yan Wang Yun-Xiang Yao Guan-Ye Li Bao-Chang Cai |
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Institution: | 1. Department of Chemistry, Nanjing Agricultural University, Nanjing 210095, PR China;2. Nanjing University of Traditional Chinese Medicine, Nanjing 210029, PR China |
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Abstract: | Aim of the studyThe aim of the present study was to evaluate the putative protective effect of 5-hydroxymethylfurfural (5-HMF) derived from processed Fructus Corni on human hepatocyte cell line (LO2) injured by hydrogen peroxide in vitro and the mechanism of its protection.Materials and methodsThe percentage of cell viability was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. The hepatocyte cell apoptosis and cell cycle were detected by flow cytometric analysis. The content of nitric oxide and caspase-3 activity were quantified spectrophotometrically by enzyme-linked immunoassay.ResultThe study showed that incubation with 5-HMF caused significant increase in the viability of LO2 cell, decrease of cell apoptosis and recovery of cell cycle in LO2 cell injured by hydrogen peroxide, which was accompanied with the decreased nitric oxide level and caspase-3 activity.ConclusionsOur data suggest that 5-HMF protects LO2 cell against damage induced by hydrogen peroxide through inhibiting effect of cell apoptosis caused by promoting S phase to G2/M phase and the decreased caspase-3 activity and nitric oxide level. 5-HMF is one of the active principles in processed Fructus Corni. |
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Keywords: | 5-HMF 5-hydroxymethylfurfural MTT 3-(4 5-dimethylthiazol-2-yl)-2 5-iphenyl tetrazolium bromide PI propidiumiodide FCM flow cytometry PBS phosphate-buffered saline |
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