中药金安粉针剂对小鼠Lewis肺癌的抑制作用及其机制探讨

目的:探讨中药金安对C₅₇BL／6J小鼠Lewis肺癌的抑制效应及其作用机制。方法:荷Lewis肺癌的C₅₇BL／6J小鼠分为生理盐水组、金安高、中、低剂量组和环磷酰胺组,通过检测各组小鼠的体重变化和抑瘤率,应用流式细胞仪和TUNEL等方法观察金安对Lewis肺癌的抑瘤机制。结果:金安高、中、低剂量组体重均明显增加,且金安高、中、低剂量组与环磷酰胺组的抑瘤率依次为45.79%、40.90%、32.48%和98.96%,流式细胞仪检测金安诱导肿瘤细胞的凋亡率分别为24.19%、14.95%和13.93%,并经统计学分析发现金安诱导肿瘤细胞的凋亡率与剂量之间存在依赖关系。结论:金安对Lewis肺癌生长具有一定的抑制作用,促进肿瘤细胞凋亡可能是金安治疗Lewis肺癌的主要作用方式。

The inhibitory effect of Chinese medicine Jinan injection on Lewis lung cancer in mice

AIM: To investigate the inhibitory effect of Chinese medicine Jinan injection(JA) on Lewis lung cancer (LLC) in mice. METHODS: The C 57 BL/6J mice with Lewis lung cancer(LLC) were divided into normal saline(NS), Jinan high dose (JAH), Jinan middle dose (JAM), Jinan low dose (JAL) and cyclophosphamide(CTX) groups. The body weight changes and inhibitory rate of LLC in each group were observed. In addition, flow cytometry and TUNEL were used to detect the anticancer mechanism of Jinan. RESULTS: The body weights were increased significantly in JA-treated groups vs CTX and the resistant rate was 45.79%, 40.90%, 32.48% and 98.96%, respectively. The apoptotic rate was 24.19%, 14.95% and 13.93% in JAH, JAM and JAL, respectively, and the Jinan induced apoptosis of LIC in a dose-dependent manner.CONCLUSION: Jinan injection inhibites the growth of LLC, and the apoptosis induction may be one of mechanisms that Jinan treates LLC in mice.