| 丝状真菌产生的柳胺酚二相代谢产物研究 |
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| 引用本文： | 马桂蕾,黄海华,孙玉明,钟大放. 丝状真菌产生的柳胺酚二相代谢产物研究[J]. 沈阳药科大学学报, 2004, 21(2): 141-144 |
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| 作者姓名： | 马桂蕾 黄海华 孙玉明 钟大放 |
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| 作者单位： | 1. 沈阳药科大学制药工程学院,辽宁,沈阳,110016
2. 沈阳药科大学药物代谢与药物动力学实验室,辽宁,沈阳,110016 |
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| 基金项目： | 国家自然科学基金资助项目 ( 3 993 0 1 80 ) |
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| 摘 要： | 目的以微生物为模型 ,对柳胺酚的二相药物代谢产物进行研究。方法选用 7株丝状真菌为转化菌株 ,利用液相色谱 质谱联用技术对药物代谢产物进行检测。结果柳胺酚经过所选真菌转化后 ,形成葡萄糖结合物和五碳糖结合物 2种二相药物代谢结合产物 ,其中葡萄糖结合物为主要产物 ,产率最高可达 83 %左右 ;且无一相药物代谢产物形成。结论利用特定的微生物可建立研究柳胺酚二相代谢适宜的体外模型。
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| 关 键 词： | 柳胺酚 微生物转化 二相药物代谢产物 小克银汉霉菌 液相色谱串联质谱分析 |
| 文章编号： | 1006-2858(2004)02-0141-04 |
| 修稿时间： | 2003-01-27 |
Study on the phase Ⅱ metabolites of the osalmide transformed by filamentous fungi |
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| MA Gui-lei,HUANG Hai-hua,SUN Yu-ming,ZHONG Da-fang. Study on the phase Ⅱ metabolites of the osalmide transformed by filamentous fungi[J]. Journal of Shenyang Pharmaceutical University, 2004, 21(2): 141-144 |
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| Authors: | MA Gui-lei HUANG Hai-hua SUN Yu-ming ZHONG Da-fang |
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| Affiliation: | MA Gui-lei1,HUANG Hai-hua1,SUN Yu-ming2,ZHONG Da-fang2 |
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| Abstract: | Objective To study phase Ⅱ metabolites of osalmide in the microbial model. Method Metabolites produced by 7 filamentous fungi screened were identified by liquid chromatography-tandem mass spectrometry (LC-MSn). Result Transformed by 7 fungi screened, the osalmide was converted to two phase Ⅱ metabolites:glucosides and pentosides,in which glucosides were the major product with the highest yield of 83%. In addition,no phase Ⅰ metabolites were produced. Conclusion The specific microorganism may serve as useful models for the study on phase Ⅱ metabolism of the osalmide. |
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| Keywords: | osalmide microbial transformation phase Ⅱ metabolites Cunninghamella species liquid chromatography-tandem mass spectrometry |
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