多烯脂肪酸对海马神经元细胞脂肪酸构成和生长的作用

目的 :　观察长链多不饱和脂肪酸花生四烯酸 (AA)和 /或二十二碳六烯酸 (DHA)对体外培养新生大鼠海马神经元脂肪酸构成和生长发育的作用。方法 :　采用无血清培养液培养新生大鼠海马神经元 ,实验组分别在对照组的无血清培养液中添加 4μmol/L AA、4μ mol/L DHA或总浓度为 4μmol/L、比例为 1∶ 2～ 1 6∶ 1的 AA和 DHA。采用毛细管气相色谱法分析神经元中脂肪酸的构成 ,NSE染色鉴定神经元并利用图像分析技术 ,测量神经元胞体大小和突起长度。结果 :　当培养液中 AA和 DHA总浓度为 4μmol/L、比例为 1∶ 2～ 1 6∶ 1 ,海马神经元中 AA百分含量、AA/DHA比值与培养液中 AA和 DHA比例均呈正相关 ;当培养液中 AA和 DHA总浓度为4μmol/L,比例为 2∶ 1或 4∶ 1时 ,海马神经元的胞体面积、最大长径、最大短径和平均突起长度均显著高于单一添加 4μ mol/L AA、4μmol/L DHA、对照组以及其余各比例组。结论 :　 AA和DHA具有显著促进体外培养海马神经元生长发育的作用

EFFECTS OF POLYENE FATTY ACID ON FATTY ACID COMPOSITION,GROWTH AND DEVELOPMENT OF HIPPOCAMPAL NEURONS

Objective: To study the effects of long chain PUFA, AA and/or DHA on the fatty acid composition, growth and development of the newborn rats hippocampal neurons in culture. Methods: The primary culture of the hippocampal neurons was carried out in vitro by using serum free medium and supplementing 4 μ mol/L AA, DHA, or AA and DHA, of which the total concentration were 4 μmol/L, the ratios of AA∶DHA from 1∶2 to 16∶1, in medium. The hippocampal neuronal fatty acid composition was analyzed by gas chromatogram, and the neuronal size and length of hippocampal neurite were measured by image analysis. Results: There were significant positive correlations between the ratios AA∶DHA in medium and the AA percentage and the ratios AA∶DHA in hippocampal neurons. The soma area? body maximum? minimum diameter and process length of hippocampal neurons in the group, which the total concentration of AA and DHA was 4 μmol/L, and the ratio AA∶DHA in medium was 2∶1 or 4∶1,were higher significantly than other groups and groups with other ratios of AA∶DHA. Conclusion: AA and DHA could promote the growth and development of hippocampal neurons in culture..