| 角膜内皮抗原及抗角膜内皮抗原抗体的提纯 |
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| 引用本文： | 唐娜. 角膜内皮抗原及抗角膜内皮抗原抗体的提纯[J]. 第一军医大学学报, 2002, 22(1): 76-78 |
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| 作者姓名： | 唐娜 |
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| 摘 要： | 目的 提纯角膜内皮抗原（corneal endothelial antigen,CEN-Ag)及抗角膜内皮抗原抗体（anti-corneal endothelial antibody,aCEN-Ab)。方法 用猪角膜内皮做抗原，上皮做对照，经离子交换层析及2B4.14.1单克隆抗体亲和层析制得纯化的CEN-Ag和角膜上皮抗原（corneal epithelial antigen,CEP-Ag)，用提纯的CEN-Ag和CEP-Ag分别免疫家兔，取血清经亲和层析得到aCEN-Ab和抗角膜上皮抗原抗体（anti-corneal epithelial antibody,aCEP-Ab)。结果 经聚丙烯酰胺凝胶电泳（SDS-PAGE）分析。抗原CEN-Ag和CEP-Ag的Mr分别为43000和53000，相应的免疫球蛋白是IgG2和IgG3。
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| 关 键 词： | 角膜内皮 抗原 抗体 层析 角膜移植 |
Purification of corneal endothelial antigen and anti-corneal endothelial antibody] |
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| Na Tang. Purification of corneal endothelial antigen and anti-corneal endothelial antibody][J]. Journal of First Military Medical University, 2002, 22(1): 76-78 |
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| Authors: | Na Tang |
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| Affiliation: | Department of Ophthalmology, First Military Medical University, Guangzhou 510515, China. |
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| Abstract: | OBJECTIVE: To explore the methods for purifying corneal endothelial antigen (CEN-Ag) and preparing anti-corneal endothelial antibody (aCEN-Ab). METHODS: Porcine corneal endothelia were obtained with the epithelia serving as control, CEN-Ag and corneal epithelial antigen (CEP-Ag) were isolated and purified by ion-exchange chromatography and affinity chromatography with 2B4.14.1 monoclonal antibody. The rabbits were immunized with the resultant CEN-Ag and CEP-Ag and their sera collected to harvest aCEN-Ab and anti-corneal epithelial antibody (aCEP-Ab) by affinity chromatography. RESULTS AND CONCLUSION: The relative molecular mass of CEN-Ag and CEP-Ag were 43 000 and 53 000 respectively as determined by SDS-PAGE, with their immunoglobulins being IgG2 and IgG3. |
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