雄激素受体对前列腺癌PC3细胞株增殖能力的影响

目的研究雄激素受体（AR）对雄激素非依赖性前列腺癌细胞株PC3增殖能力的影响。方法将含有AR的质粒（Pbabe-AR）稳定转染到PC3细胞，建立起新的细胞系PC3-AR，并用实时逆转录聚合酶链反应（Q—PCR）、蛋白免疫印迹试验（Western blot）和荧光素酶法确认AR的表达水平和功能；用MTT法、软琼脂糖凝胶实验检测AR对体外培养PC3细胞增殖的影响。结果PC3-AR细胞能够稳定表达有功能的AR；MTT实验显示PC3-AR细胞体外生长慢于PC3-v细胞，加入DHT以后效果更加明显。相对于PC3-v，PC3-AR在软琼脂糖凝胶中形成的克隆明显减少。结论AR能够降低PC3-AR细胞在体外的增殖能力。

Influence of androgen recepter to prostate cancer cell line PC3 proliferation

Objective To investigate the influence of androgen receptor （AR） to prostate cancer cell line PC3 proliferation. Methods The PC3-AR cells were established by transfecting the plasmid （Pbabe-AR） to the PC3 cells. The real-time RT-PCR, Western-blot, and reporter gene assay （Luciferase assay） were performed to detected the AR level and function in PC3-AR cells. The MTr and soft agar assay were performed to determine the in vitro growth ability of PC3 -v and PC3-AR cells. Results PC3-AR cells could express a functional AR. Compared with PC3-v cells, PC3-AR cells grew slower. After treating with DHT, PC3-AR cells grew even much slower. In soft agar assay, PC3-AR cells formed much fewer and smaller colonies than PC3-v cells. Conclusions AR can inhibit PC3 cells proliferation in vitro.