缺氧对大鼠心肌微血管内皮细胞与中性粒细胞粘附的影响

研究缺氧对心肌微血管内皮细胞与中性粒细胞粘附的影响。方法：采用细胞培养技术观察体外培养的心肌微血管ＥＣ与未活化的ＰＭＮ粘附，高倍镜计数粘附的ＰＭＮ数；采用免疫组化ＡＢＣ法检测缺氧微血管ＥＣ上细胞间粘附分子的表达。结果：心肌微血管ＥＣ缺氧２和６ｈ与ＰＭＮ的粘附显著增加，分别为对照组的１．４９倍和１．９６倍；进一步用抗ＩＣＡＭ－１和抗淋巴细胞功能和相关抗原单克隆抗体阻断实验发现，两种抗体在５μｇ／ｍｌ

Effects of hypoxia on the adherence of neutrophils to cardiac microvascular endothelial cells in rats

Objective: To study the effect of hypoxia on the neutrophils (PMN) cardiac microvascular endothelial cells(EC) adhesion. Methods: Cultured cardiac microvascular EC were divided into normal cultured group, hypoxia 2 and 6 h group, and antibodies block groups. They were incubated with unstimulated PMN respectively and adherent PMN were counted by high light microscopy. Intercellular adhesion molecule 1(ICAM 1) on hypoxia cardiac microvascular EC were immunohistochemically stained by the avidin biotin peroxidase method. Results: The adherence of rat PMN to cardiac microvascular EC was significantly stimulated by both 2 and 6 h of hypoxia(P<0.01), with increasing 1.49 and 1.96 fold respectively, and adhesion of neutrophil endothelium stimulated by hypoxia could be partly blocked by anti ICAM 1 and anti LFA 1 monoclonal antibodies. The blockage rate was about 40%. Hypoxia also increased the expression of ICAM 1 on the endothelium. Conclusion: hypoxia increases the adherence of PMN to EC, and this is involved in the increased expression of ICAM 1 on hypoxic EC. The adhesion is mediated by ICAM 1/LFA 1.