| As2O3治疗白血病的机制探讨 |
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| 引用本文： | 刘利梅,傅国辉.As2O3治疗白血病的机制探讨[J].中国现代医学杂志,2005,15(14):2117-2120. |
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| 作者姓名： | 刘利梅 傅国辉 |
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| 作者单位： | 1. 北京大学医学部,生理与病理生理学系,北京,100083
2. 哈尔滨医科大学,病理生理学教研室,黑龙江,哈尔滨,150086 |
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| 摘 要： | 目的 探讨三氧化二砷（As2O3）治疗白血病的作用机制。方法 在K562细胞中表达带3蛋白膜段结构域，经Western Blot检定其表达；通过离子转运测定验证其生物学活性；应用As2O3诱导细胞凋亡，通过电镜观察、线粒体膜电位测定和凋亡片断DNA的提取，观察As2O3对表达带3蛋白膜段结构域的K562细胞凋亡的影响。结果 表达带3蛋白膜段结构域的K562细胞的凋亡特征比未表达带3蛋白的K562细胞明显。说明表达带3蛋白膜段结构域的K562细胞对As2O3更敏感。结论 带3蛋白参与了凋亡的过程，为探讨As2O3治疗白血病的机制提供一条新的线索。
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| 关 键 词： | 三氧化二砷 细胞凋亡 带3蛋白膜段结构域 |
| 文章编号： | 1005-8982（2005）14-2117-04 |
| 收稿时间： | 2004-10-15 |
| 修稿时间： | 2004-10-15 |
Study on mechanism of arsenic trioxide treatment to leukemia |
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| LIU Li-mei,FU Guo-hui.Study on mechanism of arsenic trioxide treatment to leukemia[J].China Journal of Modern Medicine,2005,15(14):2117-2120. |
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| Authors: | LIU Li-mei FU Guo-hui |
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| Abstract: | Objective] To investigate the mechanism of Arsenic Trioxide treatment to Leukemia. Methods] We expressed band3 membrane domain in K562 cells which was identified by Western blot. The effect of As2O3 on K562 cells which expressed band3 membrane domain was observed with TEM, mitochondrial membrane potential(MMP)assay and DNA electrophoresis. Results] As2O3 induced more significant changes consistent with apoptosis in K562 cells expressed band3 membrane domain. Conclusion] The K562 cells expressed band3 membrane domain were more susceptible to As2O3, which provided a new clue to study the mechanism of arsenic trioxide treatment to leukemia. |
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| Keywords: | arsenic trioxide cell apoptosis band 3 membrane domain |
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