烟曲霉醇联合健择抑制人肺腺癌细胞和脐静脉内皮细胞VEGF及其受体的表达

目的研究烟曲霉醇（TNP-470）联合健择（Gem）对人肺腺癌细胞（A549）和人脐静脉内皮细胞（ECV-304）血管内皮生长因子（VEGF）及其受体FLT-1、KDR表达的抑制作用。方法不同浓度的Gem（10^6-10^-4mg／m1）和TNP-470（10^-3mg／ml）单用或联合处理A549和ECV-304细胞，用四甲基偶氮唑盐（MTT）比色法检测药物对细胞的毒性及增殖的影响，用半定量RT-PCR法和蛋白免疫印迹法（Western-blot）检测药物对细胞VEGF及其受体FLT-1和KDR的基因及蛋白表达的影响。结果Gem和TNP-470对两种细胞的增殖活性均有抑制作用（Gem的IC50为10^-4mg/ml；TNP-470的IC50为10^-2mg／ml），Gem联合TNP-470能明显抑制两种细胞VEGF及受体的mRNA和蛋白表达水平。结论TNP-470联合Gem能显著抑制A549、ECV-304细胞的VEGF、FIX-1和KDR的核酸和蛋白的表达，具有协同抗肿瘤作用。

TNP-470 combined with Gemcitabine inhibits the expression of vascular endothelial cell growth factor and its receptors in human pulmonary adenocarcinoma cells and umbilical vein endothelium cells

Objective To investigate the effect of TNP-470 and Gemcitabine(Gem) on the expression of VEGF, FLT-1, KDR in human pulmonary adenocarcinoma A549 cells and umbilical vein endothelium ECV-304 cells. Methods The proliferation of A549 and ECV-304 cells were measured by MTT. Semiquantitative RT-PCR and Western blot were used to examine the gene and protein expression of VEGF, FLT-1 and KDR after treating the cells with Gem and/or TNP-470 in different concentrations. Results The proliferation of A549 and ECV-304 were inhibited by Gem and TNP-470 (IC50 of Gem:10-4mg/ml, IC50 of TNP-470:10-2mg/ml). TNP-470 combined with Gem inhibited mRNA and protein expression of VEGF, FLT-1 and KDR markedly (P<0.05). Conclusion TNP-470 combined with Gemcitabine can inhibit the growth of pulmonary adenocarcinoma cells and umbilical vein endothelium cells, and their expression of VEGF, FLT-1 and KDR.