荧光原位杂交分析胰腺癌Y染色体丢失

目的 探讨男性胰腺癌Y染色体丢失与胰腺癌发生的相关性。方法 选择Y染色体长臂Yq12区域(异染色质区)DNA片段作为探针,同时选择X染色体着丝粒区(a卫星DNA)探针作为对照,通过在石蜡切片标本上进行间期细胞双色荧光原位杂交,分析15例男性胰腺癌组织Y染色体丢失的状况：结果15例胰腺癌中有10例的胰腺癌细胞存在Y染色体丢失现象,癌细胞周嗣其他细胞和正常胰腺细胞中Y染色体数目没有改变。结论 男性胰腺癌细胞中存在非随机的Y染色体丢失现象,这种高频发生的分子细胞遗传学改变很可能是胰腺癌的特征性标记之一。

Detection of Y chromosome loss by fluorescence in situ hybridization in pancreatic cancer

Objective To investigate the correlation between loss of Y chromosome and development of pancreatic cancer.Method The status of Y chromosome was analyzed by two color interphase fluorescence in situ hybridization. performed on paraffin sections of pancreatic cancer tissues from 15 Chinese males. The probes located on the heterochromatin region of chromosome Y ( maps to Yq12) and on the alpha satellite of X chromosome (maps to centromeric region) were selected for testing and as control respectively.Results The cancer cells from 10 out of the 15 pancreatic cancer patients studied showed loss of chromosome Y. The Y chromosome in the cells of surrounding non neoplastic pancreatic tissues was intact.Conclusions Loss of chromosome Y occurs non randomly in tumor cells of Chinese male patients with pancreatic cancer. This cytogenetic aberration, which happens in high frequency, may serve as one of the markers for pancreatic malignancy.