| 酵母细胞表面展示的hDAF对补体在酵母细胞表面沉积的抑制作用 |
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| 引用本文： | 郭波,郑萍,谢佩蓉,吴玉章,邹强. 酵母细胞表面展示的hDAF对补体在酵母细胞表面沉积的抑制作用[J]. 细胞与分子免疫学杂志, 2004, 20(3): 269-271 |
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| 作者姓名： | 郭波 郑萍 谢佩蓉 吴玉章 邹强 |
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| 作者单位： | 第三军医大学基础部免疫学教研室,重庆,400038 |
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| 基金项目： | 国家自然科学基金资助项目 (No .30 0 80 0 32,30 1 2 80 1 9) |
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| 摘 要： | 目的 :研究酿酒酵母细胞表面展示的人衰变加速因子 (hDAF) ,是否具有天然衰变加速因子的补体抑制活性。方法 :先用正常人血清处理酵母细胞EBY10 0 ,然后用流式细胞仪检测酵母细胞表面C5b 9复合物的沉积和hDAF在酵母细胞表面的表达。结果 :酵母细胞EBY10 0可直接活化人血清中的补体成分 ,导致酵母细胞表面C5b 9的沉积 ;而表达DAF的酵母细胞表面C5b 9的沉积明显减少。结论 :酵母细胞表面展示的DAF可正确折叠 ,并表现出DAF分子的补体抑制活性
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| 关 键 词： | 补体 衰变加速因子 酵母细胞 表面展示 |
| 文章编号： | 1007-8738(2004)03-0269-03 |
| 修稿时间： | 2003-08-16 |
Inhibitory effect of hDAF displayed on yeast cells on deposition of complement on the yeast cells |
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| GUO Bo,ZHENG Ping ,XIE Pei rong,WU Yu zhang,ZOU Qiang. Inhibitory effect of hDAF displayed on yeast cells on deposition of complement on the yeast cells[J]. Chinese journal of cellular and molecular immunology, 2004, 20(3): 269-271 |
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| Authors: | GUO Bo ZHENG Ping XIE Pei rong WU Yu zhang ZOU Qiang |
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| Affiliation: | Department of Immunology, Third Military Medical University, Chongqing 400038, China. |
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| Abstract: | AIM: To investigate whether the hDAF displayed on the surface of yeast cells can inhibit the deposition of C5b-9. METHODS: After treatment with normal human serum, deposition of human complement C5b-9 and DAF expression on yeast cells EBY100 were analyzed by flow cytometry. RESULTS: Yeast cells EBY100 could activate the complement molecules in human serum and lead to the deposition of C5b-9, while the deposition of C5b-9 on the surface of yeast cells with hDAF could significantly reduce the deposition of C5b-9. CONCLUSION: hDAF displayed on the surface of yeast cells can fold correctly and exhibit activity of complement's inhibition. |
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| Keywords: | complement decay accelerating factor yeast cell surface display |
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