| 不同配比纳米羟基磷灰石/聚己内酯复合材料细胞相容性的研究 |
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| 引用本文： | 李家锋,万美蓉,管海虹,贺文鹏,张红闯,张扬,陈丽娟. 不同配比纳米羟基磷灰石/聚己内酯复合材料细胞相容性的研究[J]. 中国美容医学, 2011, 20(2): 243-246 |
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| 作者姓名： | 李家锋 万美蓉 管海虹 贺文鹏 张红闯 张扬 陈丽娟 |
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| 作者单位： | 1. 徐州医学院附属徐州市立医院口腔颌面外科,江苏,徐州
2. 徐州医学院附属医院细胞学实验室,江苏,徐州,221002 |
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| 摘 要： | 目的：观察不同配比nPCL/HA电纺纤维取向薄膜材料的细胞相容性。方法：将人骨髓间充质干细胞（hBMSCs）体外诱导培养为成骨细胞;并经传代培养第5代的人骨髓间充质干细胞,以2×105cm2的密度与不同配比nPCL/HA电纺纤维取向薄膜支架在培养板内共培养,同时以nPCL电纺纤维非取向薄膜材料作为对照,初步观察hBMSCs在不同配比nPCL/HA支架材料上复合培养,对其细胞相容性进行评价。结果：hBMSCs与3种电纺薄膜支架材料均有细胞相容性,细胞能在不同材料表面黏附生长、分化增殖。但是PCL/HA的配比为20：1电纺纤维取向薄膜材料黏附率（35.3±2.6）%,为3中材料中黏附率最高的一种,材料表面细胞生长良好,体积变大,有伪足生长。结论：PCL/HA的配比为20：1电纺纤维取向薄膜材料,较适合作为支架材料应用于hBMSCs为种子细胞的组织工程构建。
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| 关 键 词： | 骨髓间充质干细胞 聚己内酯 羟基磷灰石 细胞相容性 |
Cellular biocompatibility of various electrospun nPCL/HA scaffold materials |
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| LI Jia-feng,WAN Mei-rong,GUAN Hai-hong,HE Wen-peng,ZHANG Hong-chuang,ZHANG Yang,CHEN Li-juan. Cellular biocompatibility of various electrospun nPCL/HA scaffold materials[J]. Chinese Journal of Aesthetic Medicine, 2011, 20(2): 243-246 |
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| Authors: | LI Jia-feng WAN Mei-rong GUAN Hai-hong HE Wen-peng ZHANG Hong-chuang ZHANG Yang CHEN Li-juan |
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| Affiliation: | LI Jia-feng1,WAN Mei-rong2,GUAN Hai-hong1,HE Wen-peng1,ZHANG Hong-chuang1,ZHANG Yang1,CHEN Li-juan1(1.Department of Oral Maxillofacial Surgery,Affiliated Xuzhou City Hospital of Xuzhou Medical College,2.Affiliated Hospital of Xuzhou Medical College,Xuzhou 221002,Jiangsu,China) |
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| Abstract: | Objective To investigate cellular biocompatibility of different nPCL/HA scaffold materials.Methods Electrostatic spinning or electrospinning is an interesting method for producing nonwoven fibers with diameters of submicrometers down to nanometers.Nanofibrous membranes were used in many biomedical applications including drug delivery,wound healing and scaffolding for tissue engineering.Novel bone-scaffolding materials were successfully fabricated by electrospinning from polycaprolactone(PCL) solutions conta... |
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| Keywords: | bone marrow stromal cells polycaprolactone hydroxypatite cellular biocompatibility |
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