加味黄芪桂枝五物汤联合甲钴胺治疗周围神经损伤的实验研究

目的：探讨加味黄芪桂枝五物汤联合甲钴胺治疗周围神经损伤的疗效.方法：将54只SD大鼠随机分为3组,每组18只,均采用左侧坐骨神经切断缝合建立坐骨神经损伤模型.造模后第3天,A组大鼠按1 mL·100 g^-1以加味黄芪桂枝五物汤灌胃,每天1次,连续4周;同时按1 mL· 100 g^-1以甲钴胺和生理盐水制成的混悬液（0.05 mg·mL^-1）灌胃,每天1次,连续4周.B组以甲钴胺和生理盐水制成的混悬液灌胃,用法、用量同A组.C组按1 mL· 100 g^-1以生理盐水灌胃,每天1次,连续4周.观察各组大鼠的一般状态,并测定坐骨神经功能指数、神经传导速度恢复率、复合肌肉诱发电位振幅恢复率、小腿三头肌湿重恢复率及神经轴突恢复率.结果：①一般状态.造模后1d所有大鼠均蜷缩伤肢,3d后跛行,切口均无感染.药物干预后第2周,3组大鼠左后小腿肌肉均有不同程度的萎缩,C组较A、B组更为明显,且C组部分大鼠出现跗关节肿胀和皮肤溃疡,多在第4周左右自行愈合.自药物干预后第4周开始,大鼠跛行逐渐改善,A组大鼠毛发光亮,运动灵活,爬行速度快;B组大鼠毛发较暗,运动较灵活,爬行速度较快;C组毛发暗淡,活动较差,反应较迟钝.②坐骨神经功能指数.药物干预后第1周、第2周、第3周及第4周坐骨神经功能指数的差异有统计学意义,即存在时间效应[（-75.61 ±8.20）,（-62.89±7.36）,（-30.89±6.14）,（-18.33±6.88）;（-75.39±7.70）,（-60.67±7.53）,（-33.11 ±6.43）,（-24.1 1±7.32）;（-80.33±6.78）,（-65.67±7.88）,（-55.22±7.26）,（-29.56±7.88）;F=2.015,P=0.000].3组大鼠坐骨神经功能指数的组间差异总体上有统计学意义,即存在分组效应（F=82.434,P=0.000）;药物干预后第3周和第4周A组的坐骨神经功能指数均高于B组和C组（P=0.004,P=0.000;P =0.023,P=0.000）.时间因素和分组因素之间存在交互效应（F=10.928,P=0.000）.③神经传导速度恢复率.3组大鼠神经?

Effect of oral application of JIAWEI HUANGQI GUIZHI WUWU decoction and mecobalamin on peripheral nerve injuries

Objective：To explore the curative effect of oral application of JIAWEI HUANGQI GUIZHI WUWU decoction and mecobalamin on peripheral nerve injuries.Methods：Fifty-four SD rats were randomly divided into 3 groups,18 rats in each group.The sciatic nerve injuries models were created through transecting and suturing the sciatic nerve at the left hind legs of rats.The rats in group A were intragastric administrated with J IAWEI HUANGQI G UIZHI WUWU decoction（1 mL/100 g）on the 3rd day after modeling,once per day for 4 consecutive weeks.Meanwhile,the rats in group A were intragastric administrated with the mecobalamin solution（0.05 mg/mL^-1 mL/100 g）,once per day for 4 consecutive weeks.The rats in group B were intragastric administrated with the mecobalamin solution（0.05 mg/mL）,which usage and dosage were same as that of group A.The rats in group C were intragastric administrated with normal saline（1 mL/100 g）,once per day for 4 consecutive weeks.After that,the general status of rats in each group were observed,and the sciatic nerve function index （SFI）,nerve conduction velocity （NCV） recovery rate,compound muscle evoked potential amplitude recovery rate,wet weight of triceps muscle（WWT） recovery rate and nerve axons recovery rate were all determined.Results：All rats curled their injured legs one day after modeling and claudication were found 3 days after modeling,and no incision infection were found.The various degrees of muscle wasting were found in the left hind legs of the rats in 3 groups on the 2nd week aftcr thc beginning of drug intervention.Compared with group A and group B,muscle wasting were more significant in group C.Meanwhile,the hock joints swelling and skin ulcers were found in several rats of group C,and most skin ulcers healed naturally on the 4th week after the beginning of drug intervention.The claudication of rats were improved gradually since the 4th week after the beginning of drug intervention.The rats in group A presented with vivid hair,swift action and fast craw