改良的豚鼠灌胃给药方法

 目的 改良豚鼠灌胃给药的方法。方法 选取清醒豚鼠40只，使其后肢站立、腹侧紧贴支持物，体位垂直不能前移，后拉两耳颊部皮毛并轻抵项部使其头后仰颈伸直，插入1 mL注射器、越过上下臼齿并压住舌根灌药；另选40只全身麻醉的豚鼠，使其侧卧头高臀低位，插入直径2 mm小儿吸痰管至胃内灌药。结果 对清醒及全麻豚鼠，连续灌胃给药7 d，80只豚鼠均顺利实现灌胃，仅需一人独立操作，无一例因操作不当引起豚鼠消化道损伤或误灌入气道死亡，成功率达100%。结论 成功改良了豚鼠的灌胃给药方法，使经口给药途径的动物实验不需因技术问题而局限于大鼠、小鼠，为进一步的药物和营养学等研究奠定了基础。

Modified gavage methods for guinea pigs

Objective To modify the method of gavage administration in guinea pigs. Methods Fourty awake guinea pigs were kept rearing on the hind legs and leaning on a vertical fixture to avoid their escaping forward. A 1 mL injector was inserted into the mouth to the depth when the molar teeth were passed. Another fourty guinea pigs under general anesthesia were reversed at trendelenburg position and a children suction tube with an outer diameter of 2 mm was inserted into the stomach. Results All of the 80 guinea pig...