|
|||||
|
|
| PLCE1通过调控p53抑制肺腺癌A549细胞凋亡 | |
| 引用本文： | 李昀,罗学平,林继宗,黄邵洪,安军,何锦园,张军航. PLCE1通过调控p53抑制肺腺癌A549细胞凋亡[J]. 中国病理生理杂志, 2015, 31(1): 119-123. DOI: 10.3969/j.issn.1000-4718.2015.01.023 |
| 作者姓名： | 李昀 罗学平 林继宗 黄邵洪 安军 何锦园 张军航 |
| 作者单位： | 1. 中山大学附属第三医院心胸外科, 广东 广州 510630; 2. 佛山市南海区人民医院心胸外科, 广东 佛山 528200; 3. 中山大学附属第三医院肝胆外科, 广东 广州 510630 |
| 基金项目： | 中山大学“985”工程项目 |
| 摘 要： | 目的:探讨磷脂酶Cε1(PLCE1)抑制肺腺癌A549细胞凋亡的作用机制。方法:选用人肺腺癌细胞株A549作为研究对象。采用real-time PCR和Western blotting法分别检测PLCE1抑制剂U-73122处理前、后肺腺癌细胞株A549中PLCE1和p53 mRNA和蛋白水平的表达;流式细胞术检测细胞凋亡。结果:肺腺癌细胞株A549高表达PLCE1,低表达p53;抑制PLCE1表达后A549细胞中p53表达上调,细胞凋亡明显增加。结论:PLCE1通过抑制肺腺癌A549细胞株中p53的表达,从而抑制A549细胞凋亡。 |
| 关 键 词： | 肺腺癌 磷脂酶Cε1 p53 细胞凋亡 甲基化 表观遗传学 |
| 收稿时间： | 2014-08-22 |
PLCE1 suppresses apoptosis of lung adenocarcinoma cells via modulating p53 expression |
|
| LI Yun,LUO Xue-ping,LIN Ji-zong,HUANG Shao-hong,AN Jun,HE Jin-yuan,ZHANG Jun-hang. PLCE1 suppresses apoptosis of lung adenocarcinoma cells via modulating p53 expression[J]. Chinese Journal of Pathophysiology, 2015, 31(1): 119-123. DOI: 10.3969/j.issn.1000-4718.2015.01.023 | |
| Authors: | LI Yun LUO Xue-ping LIN Ji-zong HUANG Shao-hong AN Jun HE Jin-yuan ZHANG Jun-hang |
| Affiliation: | 1. Department of Cardiothoracic Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China; 2. Department of Cardiothoracic Surgery, Nanhai People's Hospital, Foshan 528200, China; 3. Department of Hepatobi-liary Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China |
| Abstract: | AIM: To investigate the role of phospholipase C epsilon 1 (PLCE1) in modulating the apoptotic mechanism in lung adenocarcinoma A549 cells. METHODS: PLCE1 inhibitor U-73122 was used to suppress the expression of PLCE1. The expression of PLCE1 and p53 in A549 cells was evaluated by quantitative real-time PCR and Western blotting. Apoptosis was assessed by flow cytometry. RESULTS: A549 cells expressed high level of PLCE1 and low level of p53. Inhibition of PLCE1 markedly increased the expression of p53, and increased the apoptosis of A549 cells. CONCLUSION: PLCE1 suppresses apoptosis of A549 cells via inhibiting the expression of p53. |
| Keywords: | KEY WORDS] Lung adenocarcinoma Phospholipase C epsilon 1 p53 Apoptosis Methylation Epigenomics |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《中国病理生理杂志》浏览原始摘要信息 | |
| 点击此处可从《中国病理生理杂志》下载免费的PDF全文 | |