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| 人胃癌及癌旁组织RNA氧化损伤水平的比较及临床意义 | |
| 引用本文： | 林宪慧,余俊华,林海鸿,屠洋洋,罗顺斌,徐涛,陈统,林型城,叶孙志,郑志强,蔡剑平. 人胃癌及癌旁组织RNA氧化损伤水平的比较及临床意义[J]. 中国病理生理杂志, 2015, 31(1): 172-176. DOI: 10.3969/j.issn.1000-4718.2015.01.032 |
| 作者姓名： | 林宪慧 余俊华 林海鸿 屠洋洋 罗顺斌 徐涛 陈统 林型城 叶孙志 郑志强 蔡剑平 |
| 作者单位： | 1. 温州医科大学附属第二医院, 浙江 温州 325027; 2. 卫生部北京医院, 卫生部北京老年医学研究所, 卫生部老年医学重点实验室, 北京 100730 |
| 摘 要： | 目的:通过检测人胃癌组织及其癌旁组织中的RNA氧化损伤程度,探讨RNA氧化与胃癌发生的相关性。方法:分别利用免疫组化方法和液相-串联质谱(LC-MS/MS)方法对61例胃癌组织及其癌旁组织中8-氧鸟苷(8-oxo Gsn)进行定性和定量分析,并统计分析结果。结果:免疫组化结果检测显示8-oxo Gsn在癌旁组织中含量低,而在胃癌组织中含量明显增高,且棕色染色区域主要集中在肿瘤细胞胞浆位置。质谱检测结果显示胃癌组织中8-oxo Gsn含量较对应癌旁组织高,差异有统计学意义(P0.05)。结论:8-oxo Gsn在胃癌组织中含量增加。RNA氧化损伤可能在胃癌的发生发展过程中起重要作用。 |
| 关 键 词： | 胃肿瘤 免疫组织化学 8-氧鸟苷 液相-串联质谱 |
| 收稿时间： | 2014-09-16 |
Level and clinical significance of RNA oxidative damage in human gastric cancer and para-carcinoma tissues |
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| LIN Xian-hui,YU Jun-hua,LIN Hai-hong,TU Yang-yang,LUO Shun-bin,XU Tao,CHEN Tong,LIN Xing-cheng,YE Sun-zhi,ZHENG Zhi-qiang,CAI Jian-ping. Level and clinical significance of RNA oxidative damage in human gastric cancer and para-carcinoma tissues[J]. Chinese Journal of Pathophysiology, 2015, 31(1): 172-176. DOI: 10.3969/j.issn.1000-4718.2015.01.032 | |
| Authors: | LIN Xian-hui YU Jun-hua LIN Hai-hong TU Yang-yang LUO Shun-bin XU Tao CHEN Tong LIN Xing-cheng YE Sun-zhi ZHENG Zhi-qiang CAI Jian-ping |
| Affiliation: | 1. Department of General, Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China; 2. The Key Laboratory of Geriatrics, Beijing Hospital, Beijing Institute of Geriatrics, Ministry of Health, Beijing 100730, China |
| Abstract: | AIM: To investigate the RNA oxidative damage in human gastric cancer tissue and para-carcinoma tissue for exploring the role of RNA oxidation in the occurrence of gastric cancer. METHODS: Immunohistochemical observation and LC-MS/MS analysis were performed in 61 cases of gastric carcinoma. The position and concentration of 8-oxoguanosine (8-oxoGsn) were detected, respectively. RESULTS: The results of immunohistochemical observation showed that 8-oxoGsn was obviously up-regulated in the gastric cancer. The positive staining mainly accumulated in the cytoplasm of the tumor cells. The results of mass spectrometry showed that the level of 8-oxoGsn in the gastric cancer tissues was higher than that in the para-carcinoma tissues (P<0.05). CONCLUSION: 8-oxoGsn is up-regulated in gastric cancer. RNA oxidative damage may play important roles in the occurrence of gastric cancer. |
| Keywords: | KEY WORDS] Stomach neoplasms Immunohistochemistry 8-oxoguanosine LC-MS/MS |
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