急性肺损伤肺组织和血浆NO、ET-1变化的研究

目的 探讨急性肺损伤（ＡＬＩ）时肺组织和血浆一氧化氮（ＮＯ）、皮内素－１（ＥＴ－１）和白细胞介素８（ＩＬ－８）水平的变化和作用。方法 采用间隔２４ｈ两次注射大肠杆菌内毒素（ＬＰＳ）的方法，复制家兔内毒素急性肺损伤模型。结果 ＡＬＩ组浆、肺组织均浆及支气管肺泡灌洗液（ＢＡＬＦ）中ＮＯ、ＥＴ－１和ＩＬ－８水平显著升高（Ｐ〈０．０１），ＢＡＬＦ内中性料细胞（ＰＭＮ）明显增多，肺系数（ＬＰＬ）、肺水含量

Research of changes of nitric oxide and endothelin-1 levels in lung and plasma with acute lung injury

Objective　To explore the changes of nitric oxide(NO) and endothelin-1(ET-1) levels and its potential functions in acute lung injury(ALI).Methods　The rabbits model was induced by injecting E.Coli endotoxin twice at 24 hours interval.Results　NO,ET-1 and interleukin-8(IL-8) levels in plasma,lung tissue and bronchoalveolar lavage fluid(BALF)increased markedly (P＜0.01)in ALI group.WBC in BALF,lung index,and lung permeability index(LPI) also increased significantly (P＜0.01).In ALI group,the plasma concentrations of all NO,ET-1 and IL-8,but their temporal dynamics were different.The increase of plasma NO and IL-8 concentration reach its peak at 120 min after endotoxin injection.While plasma ET-1 concentration increased much earlier,at 60 min post-endotoxin and its plasma concentration had reached its peak level,then waned,but restored that level approach to normal rabbit at 6 hours post-injury in lung.Conclusion　NO，ET-1 and IL-8 might be an important liquit factor in different phases of ALI.All NO,ET-1 and IL-8 maybe involve in pathogenesis of ALI.