当归补血汤及其多糖对隐孢子虫感染的免疫抑制小鼠的免疫调节作用

目的：探讨当归补血汤及当归补血汤多糖对隐孢子虫感染的免疫抑制小鼠的免疫调节作用。方法：连续8d给灌服小鼠醋酸地赛米松后,于第9d经口接种1×106个隐孢子虫卵囊1次,建立隐孢子虫感染模型,再经当归补血汤及当归补血汤多糖灌胃治疗10d,计数粪便隐孢子虫卵囊,观察肠组织病理学改变,检测T细胞亚群和血清IL-2I、L-4I、FN-γ水平。并设正常对照、模型对照和西药对照组。结果：与模型组比较,当归补血汤组、当归补血汤多糖组和西药组卵囊排除数量减少（P〈0.05）,肠组织病理学改变较轻,免疫学检测发现CD4、CD4/CD8及IL-2I、L-4和IFN-γ水平明显升高（P〈0.05）。结论：当归补血汤及当归补血汤多糖通过增强机体免疫功能促进隐孢子虫感染小鼠恢复。

IMMUNE REGULATION EFFECT OF DANGGUIBUXUE DECOCTION AND ITS POLYSACCHARIDE ON THE CRYPTOSPORIDIUM-INFECTED IMMUNOSUPPRESSED MICE

Objective：To investigate the immune regulation effect of Dangguibuxue decoction and its polysaccharide on the immunosuppressed mice infected with cryptosporidium.Methods：Model mice were established by oral immunization 1×106 cryptosporidium oocyst in the 9th day one time after being fed with betamethasone acetate for＇ 8 days.Then the model mice were treated with Dangguibuxue decoction and its polysaccharide for 10 days.Cryptosporidium oocysts in faeces were daily counted and intestinal histopathological changes,T cell subsets as wellas serum IL-2,IL-4 and IFN-gamma levels were detected.In the same time normal ontrol,model control and western medicine control were set.Results：Compared with model group,decreased excluded oocysts,less intestinal histopathological changes,higher immunological detected CD4,CD4/CD8 and IL-2,IL-4 and IFN-gamma levels were found in the mice of western medicine group,Dangguibuxue decoction group and its polysaccharide group（P0.05）.Conclusion：Dangguibuxue decoction and its polysaccharide can promote the mice infected with cryptosporidium to resume by enhancing the immune function.