以类平均聚类法分析不同产地土贝母中土贝母苷甲含量及影响因素

目的测定16批不同产地土贝母中土贝母苷甲含量,确定土贝母含量高的产区,分析影响含量的因素。方法采用《中国药典》2015版土贝母药材含量方法测定土贝母苷甲;以土贝母苷甲含量为指标用类平均聚类分析法将产地来源进行分类。结果不同产地土贝母中苷甲含量在1.234%~3.174%范围内,符合药典要求;聚类分析可将16个产地分为4类,来自商洛、汉中的一类药材苷甲含量最高。结论温度和湿度可影响土贝母药材质量。温度、湿度偏高地区的土贝母药材中土贝母苷甲含量更高。

Content and influencing factors of tobeimoside I in Tubeimu from different producing areas analyzed by using group-average clustering method

Objective To identify the content of tubeimoside I in Paniculate Bolbostemma from 16 differ-ent locations and determine the high-containing locations and influencing factors.Methods The quanti-tative determination method of tobeimoside I in Tubeimu(Paniculate Bolbostemma, Rhizoma Bolbostem-atis)in Chinese Pharmacopoeia(2015 Edition)was used to determine the content of tobeimoside I in 16 batches of Tubeimu for deciding producing areas with higher content of Tubeimu and analyzing the influ-encing factors.The origin producing areas was classified by using group-average clustering analysis meth-od taken content of tobeimoside I as the index.Results The results showed that the content of tobeimo-side I was in a range from 1.234%to 3.174%in different producing areas,which was accorded with the requirement of Chinese Pharmacopoeia.The clustering analysis classified 16 producing areas into 4 groups,and content of tobeimoside I was the highest in Tubeimu material produced from Shangluo and Hanzhong.Conclusion The temperature and humidity had influence on Tubeimu material, and content of tobeimoside I was higher in the producing areas with higher temperature and humidity.