扶正剔邪搜络方对肺纤维化大鼠血管新生活动的影响

目的研究扶正剔邪搜络方对肺纤维化大鼠肺组织血管新生活动的影响,探索扶正剔邪搜络方发挥治疗作用的潜在机制。方法将健康雄性SD大鼠分为对照组、模型组和治疗组,每组32只。模型组和治疗组通过气管插管推注博莱霉素复制肺纤维化大鼠模型,对照组气管内注射等体积生理盐水。造模24 h后,治疗组按0.67 g/(kg·d)的剂量以扶正剔邪搜络方混悬液灌胃,对照组和模型组给予相同体积的生理盐水,于造模后第7、14、21、28天分别处死,每次8只,取肺组织进行HE染色、Masson染色,ELISA法检测肺组织内血管内皮生长因子(VEGF-A)及其受体(VEGFR-2)的表达情况,以免疫组织化学染色检测肺组织CD34抗原的表达情况,并通过Weidner法测微血管密度(MVD)。结果治疗组大鼠肺组织的病理改变与模型组相似,但炎症和纤维化程度均轻于同一时相模型组;治疗组大鼠各时相肺组织VEGF-A及其受体VEGF-R2的表达水平均低于同一时相模型组(P0.01或P0.05);CD34阳性细胞被染成棕黄色或褐色,治疗组大鼠微血管密度低于模型组(P0.01或P0.05)。结论扶正剔邪搜络方可以减轻博莱霉素诱导肺纤维化大鼠肺组织炎性渗出,减少胶原沉积,其机制可能与扶正剔邪搜络方降低大鼠VEGF-A及其受体VEGFR2的表达水平,抑制肺纤维化大鼠肺组织的异常血管新生活动有关。

Influence of Fuzheng Tixie Souluo Fang on angiogenesis in rats with pul-monary fibrosis

Objective To study the influence of Fuzheng Tixie Souluo Fang(Decoction of reinforcing healthy qi,eliminating pathogen and Searching collaterals,FTSF)on angiogenesis in rats with pulmonary fibrosis(PF),and investigate its latent therapeutic mechanism.Methods Health male SD rats were randomly divided into control group,model group and treatment group(each n=32).The PF model was established by intravenous injection of bleomycin via tracheal intubation in model group and treatment group,and control group was given intratracheal injection of isovolumetric normal saline.After modeling for 24 h,treatment group was given orally suspension of FTSF[0.67g/(kg· d)],and control group and model group were given isovolumetric normal saline.All rats were executed respectively at different time points(7 d,14 d,21 d and 28 d,8 each time)after modeling.The lung tissue was collected and given HE and Masson staining for detecting the expressions of vascular endothelial growth factor -A(VEGF-A) and its receptor-VEGFR-2 by using ELISA.The expression of CD34 antigen in lung tissue was detected by using immunohistochemistry(IHC)staining, and micro-vessel density was measured by using Wei-dner method.Results The pathological changes of lung tissue in treatment group were similar to those in model group,but severity of inflammation and fibrosis was milder than those in model group at the same time point.The expressions of VEGF-A and VEGF-R2 were lower in treatment group than those in model group at the same time point(P<0.01 or P<0.05).The staining of CD34 positive cells showed pale brown or brown,and micro-vessel density was lower in treatment group than that in model group(P<0.01 or P<0.05).Conclusion FTSF can relieve inflammatory exudation and collagen deposition of lung tissue induced by bleomycin in PF rats.The mechanism may be related to the decreases of VEGF-A and VEGF-R2 expressions and inhibition of abnormal angiogenic activity in lung tissue.