二甲双胍联合膈下逐瘀汤对多囊卵巢综合征大鼠炎性因子及性激素水平的影响

目的研究二甲双胍联合膈下逐瘀汤对经脱氢表雄酮诱导的多囊卵巢综合征(PCOS)模型大鼠外周血清炎性因子、性激素水平及糖脂代谢的影响。方法将69只PCOS(经脱氢表雄酮诱导)大鼠随机分为对照组(34只)和干预组(35只)。对照组大鼠每天给予二甲双胍(270 mg/kg)灌胃,干预组大鼠每天给予二甲双胍(270 mg/kg)+膈下逐瘀汤(34.5 mg/kg)灌胃,连续治疗28 d。干预结束后,用ELISA法测定2组大鼠外周血清炎性因子白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、C反应蛋白(CRP)]、血脂甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、总胆固醇(TC)]、血糖空腹血糖(FPG)、餐后2 h血糖(2 h PG)]、激素水平卵泡雌激素(FSH)、黄体生成素(LH)、雌激素(E_2)、睾酮(T)];用免疫组化法测定卵巢组织中IL-6、TNF-α蛋白表达。结果干预后2组大鼠外周血清IL-6、TNF-α、CRP、LH、LH/FSH、E_2、T、TG、LDL-C、TC、FPG、2 h PG均较治疗前有所降低(P0.05),且干预组低于对照组(P0.05);治疗后2组大鼠外周血清HDL-C、FSH较治疗前有所升高(P0.05),且干预组高于对照组(P0.05);干预后干预组大鼠的IL-6、TNF-α蛋白表达低于对照组(P0.05)。结论二甲双胍联合膈下逐瘀汤能降低PCOS大鼠外周血清炎性因子浓度,并能改善循环中血脂、血糖水平及其激素水平,且效果优于单纯使用二甲双胍。

Influence of metformin combined with Gexia Zhuyu Tang on inflammatory factors and sex hormone level in rats with polycystic ovarian syndrome

Objective To study the influence of metformin combined with Gexia Zhuyu Tang(Decoction for Dissipating Blood Stasis under Diaphragm)on serum inflammatory factors, sex hormone level and glycolipid metabolism in rat model of polycystic ovarian syndrome (PCOS) induced by dehydroepiandrosterone.Methods PCOS rats(n=69)were randomly divided into control group(n=34)and intervention group(n=35).The control group was intragastrically given metformin(270 mg/kg)and intervention group, metformin(270 mg/kg)and Gexia Zhuyu Tang(34.5 mg/kg)for continuous 28 d.The changes of serum inflammatory factorsinterleukin-6(IL-6), tumor necrosis fac-tor-α(TNF-α)and C-reactive protein(CRP)],blood fattriglyceride(TG),high-density lipoprotein-cholesterol(HDL-C),low-density lipoprotein-cholesterol(LDL-C)and total cholesterol(TC)], blood sugarfasting plasma glucose(FPG)and 2-h postprandial blood glucose(2-hPBG)],and sex hormone levelfollicle-stimulating hormone(FSH),luteinizing hormone(LH), estrogen(E2)and testosterone (T)were detected by using enzyme-linked immunosorbent assay(ELISA).The protein expressions of IL-6 and TNF-αwere detected by using immunohistochemistry technique.Results After intervention, the levels of IL-6, TNF-α, CRP, LH, LH/FSH, E2, T, TG, LDL-C, TC, FPG and 2 hPG all decreased in 2 groups(P<0.05), and they were all lower in intervention group than those in control group(P<0.05).The levels of HDL-C and FSH increased in 2 groups after intervention(P<0.05), and they were higher in intervention group than those in control group(P <0.05).The protein expressions of IL-6 and TNF-αwere lower in intervention group than those in control group(P<0.05). Conclusion Metformin combined with Gexia Zhuyu Tang can reduce the levels of peripheral serum inflammatory factors and meliorate levels of blood fat,blood sugar and hormones in PCOS rats,and the effect of which is better than that ot sinle metformin.