Characterization of a profile of the anthocyanins isolated from Vitis coignetiae Pulliat and their anti-invasive activity on HT-29 human colon cancer cells |
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| Authors: | Jeong Won Yun Won Sup Lee Min Jeong Kim Jing Nan Lu Myung Hee Kang Hoon Gu Kim Dong Chul Kim Eun Ju Choi Jin Young Choi Hae Gyeong Kim Yun-Kyoung Lee Chung Ho Ryu GonSup Kim Yung Hyun Choi Ock Jin Park Sung Chul Shin |
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| Affiliation: | 1. Department of Internal Medicine, Institute of Health Sciences, Gyeongsang National University School of Medicine, Gyeongnam Regional Cancer Center, Gyeongsang National University Hospital, Jinju 660-702, South Korea;2. Department of Pathology, Institute of Health Sciences, Gyeongsang National University School of Medicine, Gyeongnam Regional Cancer Center, Gyeongsang National University Hospital, Jinju 660-702, South Korea;3. Department of Chemistry, Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, South Korea;4. Food Technology, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, South Korea;5. School of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, South Korea;6. Department of Food and Nutrition, Hannam University Daedeok Valley Campus, Daejeon 305-811, Republic of Korea;g Department of Biochemistry, Dongeui University College of Oriental Medicine, Busan 614-052, South Korea;h Department of Biomaterial Control (BK21 Program), Dongeui University Graduate School, Busan 614-052, South Korea |
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| Abstract: | We isolated anthocyanins from fruits of Vitis coignetiae Pulliat, characterized the anthocyanin profile, and investigated the anti-invasive effects of the anthocyanins on human colon cancer cells. The anthocyanins inhibited cell invasion in a dose-dependent manner, as measured by Matrigel invasion assays, by suppression of matrix metalloproteinase (MMP)-2 and MMP-9 expression. The anti-invasive activity of the anthocyanins was associated with modulation of constitutive nuclear factor κB (NF-κB) activation. The activation of NF-κB triggered by tumor necrosis factor-α was also inhibited by the anthocyanins through suppression IκBα phosphorylation. AIMs inhibited the expression of NF-κB-regulated proteins. In conclusion, this study suggested that the anthocyanins isolated from fruits of V. coignetiae Pulliat should have anti-invasive activities on human colon cancer cells and the activities should be related to the inhibition of NF-κB-regulated proteins such as MMP-2 and MMP-9 expression through the inhibition of NF-κB activation. |
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| Keywords: | Anthocyanins, Vitis coignetiae Pulliat Matrix metalloproteinase Invasion Cancer NF-κB |
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