Natural killer cell cytotoxicity and lymphocyte perforin expression in veterans with posttraumatic stress disorder |
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Authors: | Katja Gotovac,Anđelko Vidović,Herman Vuku&scaron ić,Tomislav Krčmar,Ante Sabioncello,Sabina Rabatić,Dragan Dekaris |
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Affiliation: | 1. Institute of Immunology, Department of Research and Development, Rockefellerova 10, HR-10000 Zagreb, Croatia;2. University Hospital Center Zagreb, Department of Psychological Medicine, Kišpati?eva 12, 10000 Zagreb, Croatia;3. Croatian Academy of Science and Art, Zrinski trg 11, 10000 Zagreb, Croatia |
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Abstract: | ObjectiveTo examine the effect of posttraumatic stress disorder (PTSD) on the measures of immune function and the hypothalamic–pituitary–adrenal axis components, and to determine whether additional life stressors affect measured variables.MethodsWe simultaneously examined the natural killer cell cytotoxicity (NKCC), perforin and glucocorticoid receptor (GCR) expression in natural killer (NK) and cytotoxic T (CD8) cells, as well as serum cortisol concentration in a group of Croatian war veterans with chronic, combat-related PTSD (n = 29) and a group of healthy, age-matched men (n = 13). PTSD patients were divided into two subgroups: compensation-seeking (n = 15) and retired or compensation non-seeking (n = 14) subjects. The former includes those involved in the process of getting disability-based army retirement as an additional life stressor.ResultsNKCC was decreased in both PTSD groups when compared to controls. Impairment of NKCC could not be attributed to the perforin expression as perforin was not decreased in comparison to controls. Moreover, the increased level of perforin was recorded in NK cells of retired PTSD subjects. Both PTSD groups shared an increased relative quantity of GCR in lymphocytes, whereas no difference between the groups in the baseline levels of serum cortisol was observed.ConclusionsDiminished NKCC was not accompanied by perforin insufficiency in PTSD subjects, and other causes should be examined. An additional life stressor does not contribute considerably to either immune or endocrine system related changes. |
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Keywords: | ANCOVA, analysis of covariance BDI, Beck Depression Inventory CD, cluster of differentiation CTL, cytotoxic T lymphocytes DEX, dexamethasone DPBS, Dullbeco's phosphate buffer saline DSM, Diagnostic and Statistical Manual of Mental Disorders DTH, delayed-type hypersensitivity E:T, effector to target cell ratio FCS, fetal calf serum FDR, false discovery rate FITC, fluorescein isothiocyanate GCR, glucocorticoid receptor HPA, hypothalamic&ndash pituitary&ndash adrenal IES-R, Impact of Event Scale&mdash Revised IL, interleukin MANCOVA, multivariate analysis of covariance m.f.i., mean fluorescence intensity M-PTSD, Mississippi Scale for Combat-Related PTSD NK, natural killer NKCC, natural killer cell cytotoxicity PBMC, peripheral blood mononuclear cells PE, phycoerytrin PerCP, peridinin chlorophyll protein PTSD, posttraumatic stress disorder STAI, Spilberger State-Trait Anxiety Inventory WB, whole blood |
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